DIFFERENTIATION ANTIGENS OF MELANOCYTES AND MELANOMA - ANALYSIS OF MELANOSOME AND CELL-SURFACE MARKERS OF HUMAN PIGMENTED CELLS WITH MONOCLONAL-ANTIBODIES
DIFFERENTIATION ANTIGENS OF MELANOCYTES AND MELANOMA - ANALYSIS OF MELANOSOME AND CELL-SURFACE MARKERS OF HUMAN PIGMENTED CELLS WITH MONOCLONAL-ANTIBODIES
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DOI：
10.1111/1523-1747.ep12460906
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发表时间：
1988-04-01
影响因子：
6.5
通讯作者：
HOUGHTON, AN
中科院分区：
文献类型：
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作者：
THOMSON, TM;REAL, FX;HOUGHTON, AN
用三种识别人黑素细胞抗原的鼠单抗(MAbs)对大量培养的正常细胞、肿瘤细胞和新鲜组织进行了研究。其中两个单抗(分别命名为TA99和CF21)检测黑色素瘤抗原，而mAbC350识别细胞表面抗原。在培养的细胞中，这三种单抗与正常黑素细胞和色素黑色素瘤反应，但不与非色素黑色素瘤或其他血统的细胞反应。用mAbTA99和mAbCF21对来自大面板和组织的新鲜冰冻切片进行免疫组织化学分析，显示出仅限于着色细胞的特征反应模式。MAbC350不与任何正常组织反应。所有色素痣和原发性黑色素瘤标本以及93%的化生黑色素瘤标本至少与三种单抗中的一种呈阳性反应。62例非黑色素瘤肿瘤均未见阳性反应。TA999的表达与肿瘤的分期呈负相关。这些标记物可用于黑素细胞分化和恶变的研究、黑素细胞损伤的亚型划分，以及识别恶性黑素细胞起源的肿瘤。
Three mouse monoclonal antibodies (mAbs) recognizing antigens of human melanocytic cells were used to study a large panel of cultured normal and tumor cells and fresh tissues. Two of the monoclonal antibodies (designated TA99 and CF21) detect melanosomal antigens, whereas mAbC350 recognizes a cell surface antigen. Among cultured cells the three mAbs reacted with normal melanocytes and pigmented melanomas, but not with nonpigmented melanomas or cells of other lineage. Immunohistochemical assays using mAbTA99 and mAbCF21 on frese-frozen sections from a large panel and tissues revealed a characteristic pattern of reactivity restricted to pigmented cells. mAbC350 did not react with any normal tissues. All nevi and primary melanoma specimens and 93% of metastic melanomas were reactive with at least one of three monoclonal antibodies. No reactivity was found with 62 nonmelanoma tumors. An inverse correlation was observed between TA999 expression and stage of tumor progression. These markers are useful for studies of melanocyte differentiation and malignant transformation, subsetting of melanocytic lesions, and identification of tumors of malanocytic origin.