Siboglinid-bacteria endosymbiosis
Siboglinid-bacteria endosymbiosis
复制标题
西博列尼-细菌内共生
DOI：
--
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发表时间：
2008
影响因子：
--
通讯作者：
K. Halanych
中科院分区：
文献类型：
--
作者：
D. Thornhill;K. Fielman;S. R. Santos;K. Halanych
Siboglinid蠕虫是一群无胆的海洋环节动物，其营养依赖于内共生细菌。1-2已知的siboglinids有4个主要类群，包括veestimentiferans、Osedax spp、frenulate和moniliferans。3-5关于与链状或单胞siboglinids相关的细菌内共生体的多样性知之甚少。考虑到西博格列酮的全球分布，这种知识的缺乏令人惊讶；这个系统可能是深海中最常见的共生系统之一。至少有三个不同的内共生γ-变形菌门分支与sibogllinid环节动物相关Frenulates包含一个γ -变形细菌分支，它不同于veestimentiferans和monoliferans的硫营养细菌以及Osedax sp的异养细菌。6-7我们还讨论了未来siboglinid研究的优先事项以及超越描述性研究的必要性。激光捕获显微解剖（LCM）是一种很有前途的新方法，可以精确切除感兴趣的组织区域这种方法，当与分子和基因组技术（如使用焦磷酸测序技术的表达序列标签调查）一起使用时，将有可能对这些共生的生理过程和机制进行研究。此外，采用比较方法，使用不同的siboglinids组，如蠕虫窝藏硫营养型和甲烷营养型内共生体，可能会对Siboglinidae的生态学和进化产生相当大的见解。
Siboglinid worms are a group of gutless marine annelids which are nutritionally dependent upon endosymbiotic bacteria.1-2 Four major groups of siboglinids are known including vestimentiferans, Osedax spp., frenulates, and moniliferans.3-5 Very little is known about the diversity of bacterial endosymbionts associated with frenulate or monoliferan siboglinids. This lack of knowledge is surprising considering the global distribution of siboglinids; this system is likely among the most common symbioses in the deep sea. At least three distinct clades of endosymbiotic γ-proteobacteria associate with siboglinid annelids.6 Frenulates harbor a clade of γ–proteobacteria that are divergent from both the thiotrophic bacteria of vestimentiferans and monoliferans as well as the heterotrophic bacteria of Osedax spp.6-7 We also discuss priorities for future siboglinid research and the need to move beyond descriptive studies. A promising new method, laser-capture microdissection (LCM), allows for the precise excision of tissue regions of interest.8 This method, when used in concert with molecular and genomic techniques, such as Expressed Sequence Tag surveys using pyrosequencing technology, will likely enable investigations into physiological processes and mechanisms in these symbioses. Furthermore, adopting a comparative approach using different siboglinid groups, such as worms harboring thiotrophic versus methanotrophic endosymbionts, may yield considerable insight into the ecology and evolution of the Siboglinidae.
DOI：
10.1073/pnas.95.17.9962
发表时间：
1998-08-18
影响因子：
11.1
作者：
Peek, AS;Feldman, RA;Vrijenhoek, RC
通讯作者：
Vrijenhoek, RC