Quantification of single-cell nanoparticle concentrations and the distribution of these concentrations in cell population.
Quantification of single-cell nanoparticle concentrations and the distribution of these concentrations in cell population.
复制标题
单细胞纳米颗粒浓度的定量以及这些浓度在细胞群中的分布。
DOI：
10.1098/rsif.2013.1152
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Sitharaman,Balaji
中科院分区：
文献类型：
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作者：
Rashkow,JasonT;Patel,SunnyC;Tappero,Ryan;Sitharaman,Balaji
细胞中纳米颗粒摄取的定量对于细胞成像和治疗中的许多应用是必要的。在此，同步辐射X射线荧光（SXRF）显微镜，一个有前途的工具，以量化植物和动物细胞中的元素，被用来量化和表征二氧化钛（TiO 2）纳米球摄取的单细胞群体中的分布。将这些结果与通过广泛使用的电感耦合等离子体质谱法（ICP-MS）获得的每个细胞的平均纳米颗粒浓度进行比较。结果表明，与ICP-MS相比，通过SXRF定量的每个细胞的纳米颗粒浓度大一到两个数量级。SXRF结果还表明每个细胞的纳米颗粒浓度呈高斯分布。结果表明，与单细胞分析领域相关的问题，从大群体平均值确定物理参数的方法的局限性导致潜在的误导性信息，以及缺乏有关细胞异质性的任何信息，对于细胞群体中纳米颗粒的定量同样相关。
Quantification of nanoparticle uptake into cells is necessary for numerous applications in cellular imaging and therapy. Herein, synchrotron X-ray fluorescence (SXRF) microscopy, a promising tool to quantify elements in plant and animal cells, was employed to quantify and characterize the distribution of titanium dioxide (TiO2) nanosphere uptake in a population of single cells. These results were compared with average nanoparticle concentrations per cell obtained by widely used inductively coupled plasma mass spectrometry (ICP-MS). The results show that nanoparticle concentrations per cell quantified by SXRF were of one to two orders of magnitude greater compared with ICP-MS. The SXRF results also indicate a Gaussian distribution of the nanoparticle concentration per cell. The results suggest that issues relevant to the field of single-cell analysis, the limitation of methods to determine physical parameters from large population averages leading to potentially misleading information and the lack of any information about the cellular heterogeneity are equally relevant for quantification of nanoparticles in cell populations.