Localization of connective tissue growth factor during the period of embryo implantation in the mouse.
Localization of connective tissue growth factor during the period of embryo implantation in the mouse.
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DOI：
10.1095/biolreprod59.5.1207
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发表时间：
1998-11
影响因子：
3.6
通讯作者：
G. A. Surveyor;Amy K. Wilson;D. Brigstock
中科院分区：
文献类型：
--
作者：
G. A. Surveyor;Amy K. Wilson;D. Brigstock
结缔组织生长因子（CTGF）在生殖功能中的作用已从最近的研究表明，在猪。为了扩展这些发现，我们分析了CTGF在小鼠动情周期和妊娠早期的免疫组织化学定位。在动情间期和动情前期早期，CTGF定位于高水平的管腔和腺子宫上皮细胞和基质或肌层中的水平要低得多。CTGF的上皮表达在发情期显著降低。在妊娠第1.5-3.5天，CTGF主要定位于子宫上皮细胞，其在第4.5天显示CTGF水平显著降低。在第5.5天和第6.5天，CTGF以高水平存在于子宫蜕膜细胞中。在第4.5天，在植入前胚胎的滋养外胚层和内细胞团中检测到CTGF，并在第5.5-6.5天优先定位于胚胎内胚层和中胚层。在子宫内膜提取物和子宫腔冲洗液中存在多种团块形式的CTGF（Mr 14000 - 38000）。总的来说，这些数据支持CTGF在动情周期和早期妊娠期间子宫细胞生长、迁移、粘附和细胞外基质产生以及胚胎早期发育中的作用。
A role for connective tissue growth factor (CTGF) in reproductive function has been suggested from recent studies in the pig. To extend these findings, we have analyzed the immunohistochemical localization of CTGF during the estrous cycle and early pregnancy in mice. During the diestrous and early proestrous stages, CTGF was localized at high levels to both luminal and glandular uterine epithelial cells and at much lower levels in the stroma or myometrium. Epithelial expression of CTGF was considerably reduced at estrus. On Days 1.5-3.5 of pregnancy, CTGF was localized mainly to the uterine epithelial cells, which showed a substantially reduced level of CTGF on Day 4.5. On Days 5.5 and 6.5, CTGF was present at high levels in uterine decidual cells. CTGF was detected in the trophectoderm and inner cell mass of the preimplantation embryo on Day 4.5 and became preferentially localized to embryonic endoderm and mesoderm on Days 5.5-6.5. Multiple mass forms of CTGF (Mr 14 000-38 000) were present in endometrial extracts and uterine luminal flushings. Collectively, these data support a role for CTGF in uterine cell growth, migration, adhesion, and extracellular matrix production during the estrous cycle and early pregnancy, as well as in early development of the embryo.