Hydration of single-stranded DNA in water studied by infrared spectroscopy
Hydration of single-stranded DNA in water studied by infrared spectroscopy
复制标题
DOI：
10.1016/j.cplett.2006.12.102
复制
发表时间：
2007-02-27
影响因子：
2.8
通讯作者：
Niwano, Michio
中科院分区：
文献类型：
--
作者：
Miyamoto, Ko-ichiro;Onodera, Kota;Niwano, Michio
用红外吸收光谱研究了胸腺嘧啶(dt(10))和腺嘌呤(da(10))的单链DNA(寡核苷酸)在重水中的水化反应。红外吸收数据与从头计算结果的比较清楚地表明，水分子结合在胸腺嘧啶碱基的C=O和N-H键上，导致C=O伸缩振动模红移，并且水分子与胸腺嘧啶碱基结合是位置特异性的。我们发现，水合作用导致腺嘌呤碱基的环变形模式发生蓝移。(C)2007 Elsevier B.V.保留所有权利。
Hydration of single-stranded DNAs (oligonucleotides) of thymine (dT(10)) and adenine (dA(10)) in heavy water has been investigated using infrared absorption spectroscopy in the geometry of multiple internal reflection. Comparison of infrared absorption data with results of ab initio cluster calculation clearly indicates that water molecules are bound to the C=O and N-H bonds of the thymine base, giving rise to a red-shift of the C=O stretching vibration modes, and that the water molecule is site-specifically bound to the thymine base. We find that hydration induces a blue-shift of the ring deformation mode of the adenine base. (c) 2007 Elsevier B.V. All rights reserved.