Sulfur-Functionalized Fullerene Nanoparticle as an Inhibitor and Eliminator Agent on Pseudomonas aeruginosa Biofilm and Expression of toxA Gene
Sulfur-Functionalized Fullerene Nanoparticle as an Inhibitor and Eliminator Agent on Pseudomonas aeruginosa Biofilm and Expression of toxA Gene
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DOI：
10.1089/mdr.2018.0008
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发表时间：
2018-11-21
影响因子：
2.6
通讯作者：
Fooladi, Abbas Ali Imani
中科院分区：
文献类型：
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作者：
Darabpour, Esmaeil;Doroodmand, Mohammad Mahdi;Fooladi, Abbas Ali Imani
在过去的十年中，基于纳米技术的治疗平台已经被导向开发具有独特特性的纳米颗粒以对抗生物膜。在这项研究中，我们评估了硫功能化富勒烯纳米粒子（SFF Nps）对铜绿假单胞菌的生物膜活性，并分析了这种纳米粒子对外毒素A（toxA）基因表达的影响。采用化学气相沉积法制备了功能化富勒烯。我们评估了SFF Nps抑制生物膜形成和根除预先形成的生物膜的潜力。此外，该纳米颗粒对toxA基因的表达的影响进行了研究，通过实时PCR。SFF Nps的最小生物膜抑制浓度为lmg/mL。SFF Nps在年轻（24和48小时）和老年（72和96小时）生物膜上的最小生物膜根除浓度分别为2和4 mg/mL。场发射电子扫描显微镜图像证实了SFF Nps根除铜绿假单胞菌生物膜的强大能力。在SFF Nps存在下，toxA的表达下调。总之，考虑到SFF Nps杀死铜绿假单胞菌生物膜并下调外毒素A表达的能力，该纳米颗粒可用于治疗慢性和急性铜绿假单胞菌感染。
Over the last decade, nanotechnology-based therapeutic platforms have been directed toward developing nanoparticles with unique properties to combat biofilms. In this study, we evaluated the antibiofilm activity of the sulfur-functionalized fullerene nanoparticles (SFF Nps) against Pseudomonas aeruginosa and also analyzed the effect of this nanoparticle on the expression of exotoxin A (toxA) gene. The functionalized fullerenes were prepared by chemical vapor deposition method. We assessed the potential of SFF Nps to inhibit biofilm formation and eradicate preformed biofilms. Also, the effect of this nanoparticle on the expression of toxA gene was investigated by real-time PCR. The minimum biofilm inhibitory concentration of SFF Nps was 1 mg/mL. The minimum biofilm-eradication concentration of SFF Nps on the young (24- and 48-hr old) and older (72- and 96-hr old) biofilms was 2 and 4 mg/mL, respectively. Field emission electron scanning microscopy images confirmed the potent ability of SFF Nps to eradicate biofilm of P. aeruginosa. The expression of toxA was downregulated in the presence of SFF Nps. In conclusion, considering the ability of SFF Nps to kill P. aeruginosa biofilm and downregulate the expression of exotoxin A, this nanoparticle can be used for treatment of both chronic and acute P. aeruginosa infections.