In vitro biochemical assays to monitor rhodopsin function.
In vitro biochemical assays to monitor rhodopsin function.
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DOI：
10.1007/978-1-61779-848-1_11
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发表时间：
2012-01-01
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影响因子：
--
通讯作者：
Gross, Alecia K
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文献类型：
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作者：
Sammons, Joshua;Gross, Alecia K
视紫红质是负责启动视觉转导级联的昏暗光感受器。在黑暗中，它的活性很低，而光激活催化其g蛋白转导蛋白的激活。重置视紫红质和光转导级联的第一步涉及到由视紫红质激酶磷酸化的光活性视紫红质。在这里，我们描述了检测视紫红质或视紫红质突变体的功能。
Rhodopsin is the dim-light photoreceptor responsible for initiation of the visual transduction cascade. In the dark its activity is very low, while light activation catalyzes the activation of its G-protein transducin. The first step in resetting rhodopsin and the phototransduction cascade involves the phosphorylation of light-active rhodopsin by rhodopsin kinase. Here, we describe assays to monitor the function of rhodopsin or rhodopsin mutants.