INORGANIC PYROPHOSPHATE IS LOCATED PRIMARILY IN THE MITOCHONDRIA OF THE HEPATOCYTE AND INCREASES IN PARALLEL WITH THE DECREASE IN LIGHT-SCATTERING INDUCED BY GLUCONEOGENIC HORMONES, BUTYRATE AND IONOPHORE A23187
INORGANIC PYROPHOSPHATE IS LOCATED PRIMARILY IN THE MITOCHONDRIA OF THE HEPATOCYTE AND INCREASES IN PARALLEL WITH THE DECREASE IN LIGHT-SCATTERING INDUCED BY GLUCONEOGENIC HORMONES, BUTYRATE AND IONOPHORE A23187
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DOI：
10.1042/bj2540379
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发表时间：
1988-09-01
影响因子：
4.1
通讯作者：
HALESTRAP, AP
中科院分区：
文献类型：
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作者：
DAVIDSON, AM;HALESTRAP, AP
1.研究了不同激素对大鼠肝细胞PPI含量和光散射的影响。2.PPI的基础含量约为130pmoL/mg细胞蛋白，在添加激素后增加，同时我们先前观察到的光散射减少[Quinlan，Thomas，Armston和Halestrap(19830 Biochem)]。J.214,295-404]。3.激动剂对PPI含量的平均增加(以pmol/mg蛋白质计)为：0.1um-M-高血糖素，25；20-m-苯肾上腺素，30；25 nM-加压素，127；高血糖素+苯肾上腺素，115；高血糖素+加压素，382；100-M-ADP，50；15-M-A23187，72；1 mM-丁酸盐，80.4。在无细胞外Ca~(2+)存在的情况下，加压素对肝细胞的PPI含量和光散射几乎没有影响。5.在PPI不超过300pmol/mg蛋白质的情况下，PPI含量的增加幅度与光散射的减少程度相关，与刺激剂无关。在该值以上，几乎没有观察到光散射的额外变化。6.亚细胞分级显示，对照组和刺激组细胞PPI的90%以上位于线粒体内。7.这些数据支持以前使用分离的肝脏线粒体的实验的结论[Davidson和Halestrap(1987)Biochem]。J.246,715-723]激素通过钙离子诱导的基质增加线粒体基质体积[PPI]。8.进一步提出，线粒体[PPI]的这种增加允许ADP进入线粒体以换取PPI，因此是激素处理后观察到的线粒体腺嘌呤核苷酸总量增加的原因。
1. The effects of a variety of hormones on the PPi content and light-scattering of isolated rat liver cells was studied. 2. The basal PPi content was about 130 pmol/mg of cell proein, and increased after hormone addition, in parallel with a decrease in light-scattering which we have observed previously [Quinlan, Thomas, Armston and Halestrap (19830 Biochem. J. 214, 295-404]. 3. The mean increases in PPi content with the agonists shown (as pmol/mg of protein) were: 0.1 .mu.M-glucagon, 25; 20 .mu.M-phenylephrine, 30; 25 nM-vasopressin, 127; glucagon + phenylephrine, 115; glucagon + vasopressin, 382; 100 .mu.M-ADP, 50; 15 .mu.M-A23187, 72; 1 mM-butyrate, 80.4. In the absence of extracellular Ca2+, vasopressin had little effect on either the PPi content or the light-scattering of hepatocytes. 5. The magnitude of the increase in PPi content correlated with that of the decrease in light-scattering irrespective of the stimulating agent, provided that the PPi did not exceed 300 pmol/mg of protein. Above this value little additional change in light-scattering was observed. 6. Subcellular fractionation showed that over 90% of the cellular PPi was intramitochondrial in both control and stimulated cells. 7. The data support the conclusions of previous experiments using isolated liver mitochondria [Davidson and Halestrap (1987) Biochem. J. 246, 715-723] that hormones increase the mitochondrial matrix volume through a Ca2+-induced rise in matrix [PPi]. 8. It is further proposed that this increase in mitochondrial [PPi] allows entry of ADP into the mitochondria in exchange for PPi and is therefore responsible for the increase in total mitochondrial adenine nucleotides observed after hormone treatment.