Comparison of radioimmunoprecipitation versus antigen-specific assays for identification of myositis-specific autoantibodies in dermatomyositis patients
Comparison of radioimmunoprecipitation versus antigen-specific assays for identification of myositis-specific autoantibodies in dermatomyositis patients
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DOI：
10.3109/14397595.2014.896494
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发表时间：
2014-11-01
影响因子：
2.2
通讯作者：
Song, Yeong Wook
中科院分区：
文献类型：
--
作者：
Kang, Eun Ha;Kuwana, Masataka;Song, Yeong Wook
背景。目的：确认韩国皮肌炎（DM）患者放射免疫沉淀法检测到的140-kDa（抗p140）或155/140-kDa多肽（抗p155/140）自身抗体的抗原特异性，探讨两种MSA与DM临床特征的关系。采用酶联免疫吸附法（用于抗mda5抗体）和免疫印迹法（用于抗mj /NXP-2和抗tif -1抗体）检测了17例在我们之前的研究中发现有抗p140抗体（n = 9）或抗p155/140抗体（n = 8）的典型DM患者的血清样本。.Results抗体)。9例抗p140抗体阳性患者中有7例存在抗mda5抗体。9人中有2人有抗mj /NXP-2抗体，无间质性肺疾病（ILD）。8例抗p155/140抗体阳性患者均有抗tif -1。抗体。Anti-TIF-1。1例HIV感染非霍奇金淋巴瘤患者同时检测到抗p155/140抗体和抗mda5抗体。抗mda5抗体与快速进展性ILD和抗tif -1之间的关系。抗体和肿瘤相关DM的相关性显著。虽然放射免疫沉淀看起来仍然是一个很好的筛选工具，但抗原特异性测定法的确认似乎是必要的。抗mda5与快速进展性ILD之间以及抗tif -1之间的关系。和癌症相关糖尿病在韩国糖尿病患者中得到证实。
Background. To confirm the antigen specificities of autoantibodies that precipitate 140-kDa (anti-p140) or 155/140-kDa polypeptides (anti-p155/140) previously identified by radioimmunoprecipitation in Korean patients with dermatomyositis (DM) and to look into the relationship between each MSA and clinical features of DM.Methods. Seventeen serum samples of classic DM patients who had been found to have either anti-p140 antibodies (n = 9) or anti-p155/140 (n = 8) antibodies in our previous study were examined using enzyme-linked immunosorbent assay (for anti-MDA5 antibodies) and immunoblotting (for anti-MJ/NXP-2 and anti-TIF-1. antibodies).Results. Seven out of nine anti-p140 antibody positive patients were found to have anti-MDA5 antibodies. Two out of nine had anti-MJ/NXP-2 antibodies with no interstitial lung disease (ILD). All eight anti-p155/140 antibody positive patients were found to have anti-TIF-1. antibodies. Anti-TIF-1. and anti-MDA5 antibodies were simultaneously detected in one patient with anti-p155/140 antibody, who suffered HIV infection and non-Hodgkin's lymphoma. The associations between anti-MDA5 antibody and rapidly progressive ILD and between anti-TIF-1. antibody and cancer-associated DM were confirmed to be significant.Conclusions. Although radioimmunoprecipitation still looks to be a good screening tool, confirmation with antigen-specific assays seems mandatory. The associations between anti-MDA5 and rapidly progressive ILD and between anti-TIF-1. and cancer-associated DM were confirmed in Korean patients with DM.