Determination of amino acid enantiomers in pharmaceuticals by reversed-phase high-performance liquid chromatography
Determination of amino acid enantiomers in pharmaceuticals by reversed-phase high-performance liquid chromatography
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DOI：
10.1023/b:janc.0000011669.08932.d8
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发表时间：
2004-01-01
影响因子：
1.1
通讯作者：
Shpigun, OA
中科院分区：
文献类型：
--
作者：
Chernobrovkin, MG;Anan'eva, IA;Shpigun, OA
采用邻苯二甲醛/N-乙酰基-L-半胱氨酸（OPA/NAC）试剂柱前衍生化，通过反相高效液相色谱法测定氨基酸对映体。以四种氨基酸为例，研究了洗脱液的组成和pH值对衍生物分离的影响。结果发现，最高的选择性和效率的分离OPA/NAC衍生物的氨基酸是达到与使用的洗脱剂甲醇-0.01 M Na 2 HPO 4（pH6.0）。选择了最佳的移动的相组成和梯度洗脱条件，用于分离20种氨基酸衍生物的混合物。建立了肠外营养制剂中氨基酸对映体的测定方法。该方法用于测定多胺制剂中精氨酸、丙氨酸、蛋氨酸、苯丙氨酸和亮氨酸的D-异构体。
Precolumn derivatization with the reagent o-phthalic aldehyde/N-acetyl-L-cysteine (OPA/NAC) was used for the determination of amino acid enantiomers by reversed-phase high-performance liquid chromatography. The influence of the composition and pH of the eluent on the separation of the resulting derivatives was studied with the example of four amino acids. It was found that the highest selectivity and efficiency of the separation of OPA/NAC derivatives of amino acids is attained with the use of the eluent methanol-0.01 M Na2HPO4 (pH 6.0). The optimum composition of the mobile phase and conditions of the gradient elution were selected for the separation of a mixture of 20 amino acid derivatives. A procedure was developed for the determination of amino acid enantiomers in parenteral nutrition preparations. The procedure was used for the determination of D-isomers of arginine, alanine, methionine, phenylalanine, and leucine in the preparation Polyamine.