Fibrin in peritonitis. V. Fibrin inhibits phagocytic killing of Escherichia coli by human polymorphonuclear leukocytes.
Fibrin in peritonitis. V. Fibrin inhibits phagocytic killing of Escherichia coli by human polymorphonuclear leukocytes.
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腹膜炎中的纤维蛋白。
DOI：
10.1097/00000658-198604000-00013
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发表时间：
1986
影响因子：
9
通讯作者：
Simmons,RL
中科院分区：
文献类型：
--
作者：
Rotstein,OD;Pruett,TL;Simmons,RL
由腹膜炎引起的纤维素沉积被认为是一种重要的局部防御机制，因为它隔离和阻挡细菌溢出。然而，在大鼠腹膜炎模型中，纤维蛋白已被证明易导致残余脓肿形成。为了检查这种作用的潜在机制，在体外测试了纤维蛋白对中性粒细胞功能的抑制作用。在所有测试浓度（50-1000 mg/dl）下，纤维蛋白显著损害中性粒细胞杀死大肠杆菌的能力。这种抑制作用以剂量依赖性方式发生，在最高试验浓度下几乎完全防止了杀灭。进一步的研究表明，预先暴露于纤维蛋白不会降低中性粒细胞的去炎性能力，进行呼吸爆发，或杀死E。表明纤维蛋白对中性粒细胞的正常杀微生物功能没有造成不可逆的损害。然而，在生理浓度下，纤维蛋白显著损害放射性标记的E.杆菌这些数据支持这样的概念，即细菌的吞噬作用被纤维蛋白中的嗜中性粒细胞损害。因此，即使有足够数量的正常白细胞存在，污染的纤维蛋白也可以作为腹膜炎后残留脓肿形成的病灶。
Fibrin deposition initiated by peritonitis is thought to be an important local defense mechanism because it sequesters and walls off bacterial spillage. However, fibrin has been shown to predispose to residual abscess formation in a rat peritonitis model. To examine the potential mechanisms of this effect, fibrin was tested in vitro for its inhibitory effect on neutrophil function. At all concentrations tested (50–1000 mg/dl), fibrin significantly impaired the ability of neutrophils to kill Escherichia coli. This inhibition occurred in a dose dependent fashion with almost complete prevention of killing at the highest concentration tested. Further studies showed that pre-exposure to fibrin did not reduce the neutrophil's ability to degranulate, undergo a respiratory burst, or kill E. coli, indicating that fibrin did not cause irreversible damage to the normal microbicidal functions of the neutrophil. However, fibrin, at physiologic concentrations, significantly impaired phagocytosis of radiolabeled E. coli. The data support the concept that phagocytosis of bacteria is impaired by neutrophils enmeshed in fibrin. Thus, contaminated fibrin could act as a nidus for residual abscesses formation following peritonitis even if an adequate number of normal leukocytes were present.