The effect of exercise training on oxidative damage of lipids, proteins, and DNA in rat skeletal muscle:: Evidence for beneficial outcomes
The effect of exercise training on oxidative damage of lipids, proteins, and DNA in rat skeletal muscle:: Evidence for beneficial outcomes
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DOI：
10.1016/s0891-5849(99)00038-6
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发表时间：
1999-07-01
影响因子：
7.4
通讯作者：
Goto, S
中科院分区：
文献类型：
--
作者：
Radák, Z;Kaneko, T;Goto, S
适度的日常锻炼对健康有益，可以降低许多与年龄有关的疾病的风险。产生这些效应的分子机制尚不清楚。相反，有人声称，某些类型的长时间体力消耗对健康有害，因为通过增加氧消耗而过度产生活性氧物质。使用两个年龄组的大鼠，年轻（4周）和中年（14个月），我们研究了长期游泳训练对磷脂，蛋白质和DNA的氧化状态的影响。硫代巴比妥酸反应物质和4-羟基壬烯醛蛋白加合物的浓度在两个年龄组的运动和nonexercised动物之间的腓肠肌没有差异。运动大鼠中分子量约为29 KDa的蛋白质的羰基化程度和核DNA中8-羟基脱氧鸟苷的量小于久坐动物（p <0.05）。DT-心肌黄酶（C1：29.3 +/- 1.9; C2：36.1 +/- 2.6; E1：27.2 +/- 1.3; C2：33.4 +/- 2.9 nmol/mg蛋白质）和蛋白酶体（一种氧化修饰蛋白的主要蛋白水解酶）的活性在两个年龄组的运动动物中显著较高（p <0.05）。适度耐力运动引起的抗氧化应激的适应性反应构成了运动的有益效应。(C)1999 Elsevier Science Inc.
Moderate daily exercise is known to be beneficial to health, reducing risks of a number of age-related disorders. Molecular mechanisms that bring about these effects are not clear. In contrast, it has been claimed that some types of prolonged physical exertion are detrimental to health because active oxygen species are generated excessively by enhanced oxygen consumption. Using two age groups of rats, young (4 week) and middle aged (14 months), we investigated the effects of long-term swimming training on the oxidative status of phospholipids, proteins, and DNA. The concentration of thiobarbituric acid reactive substances and 4-hydroxynonenal protein adducts did not differ in the gastrocnemius muscle between exercised and nonexercised animals in the two age groups. The extent of carbonylation in a protein of molecular weight around 29 KDa and the amount of 8-hydroxydeoxyguanosine in nuclear DNA were smaller (p < .05) in the exercised rats than in the sedentary animals. Activities of DT-diaphorase (C1: 29.3 +/- 1.9; C2: 36.1 +/- 2.6; E1: 27.2 +/- 1.3; C2: 33.4 +/- 2.9 nmol/mg protein) and proteasome, a major proteolytic enzyme for oxidatively modified proteins were significantly higher in the exercised animals of both age groups (p < .05). The adaptive response against oxidative stress induced by moderate endurance exercise constitutes a beneficial effect of exercise. (C) 1999 Elsevier Science Inc.