K⁺ dynamics in ischemic rat brain in vivo by ⁸⁷Rb MRI at 7 T.
K⁺ dynamics in ischemic rat brain in vivo by ⁸⁷Rb MRI at 7 T.
复制标题
DOI：
10.1002/nbm.1652
复制
发表时间：
2011-08
影响因子：
2.9
通讯作者：
Jones, Stephen C.
中科院分区：
文献类型：
--
作者：
Yushmanov, Victor E.;Kharlamov, Alexander;Ibrahim, Tamer S.;Zhao, Tiejun;Boada, Fernando E.;Jones, Stephen C.
The aims of the present study were 1) to perform the first 87Rb MRI in live rats with a focal ischemic stroke; and 2) to test the hypothesis that K+ egress from the brain in this model is quantifiable in individual animals by the high field (7 T) K/Rb substitution MRI. Rats pre-loaded with dietary Rb+ (resulting in replacement ratios Rb/(K+Rb) of 0.1 to 0.2 in the brain) were subjected to permanent occlusion of the middle cerebral artery, and 87Rb MRI was implemented with 13-min temporal resolution using a dedicated radiofrequency coil and a spiral ultrashort-TE sequence (TR/TE of 3/0.07 ms). The ischemic core was localized by apparent diffusion coefficient mapping, by microtubule-associated protein-2 immunohistochemistry, and by changes in surface reflectivity. [K], [Na] and [Rb] were independently determined in the micropunched samples by post-mortem flame photometry. Both techniques were generally in agreement in the non-ischemic cortex, however, the MRI-assessed [K++Rb+] drop in ischemic brain was less pronounced (average efflux rate of 4.8 ± 0.2 nEq/mm3/h, vs. 10 ± 1 nEq/mm3/h by flame photometry, P < 0.0001). The use of higher field gradients for better spatial resolution and, hence, more accurate quantitation, is suggested.
登录
查看更多内容
影响因子：
3.3
作者：
Yushmanov, Victor E.;Kharlamov, Alexander;Jones, Stephen C.
通讯作者：
Jones, Stephen C.
影响因子：
8.3
作者：
SCHUIER, FJ;HOSSMANN, KA
通讯作者：
HOSSMANN, KA
影响因子：
2
作者：
Kharlamov, A;Jones, SC;Kim, DK
通讯作者：
Kim, DK
影响因子：
2.9
作者：
Kupriyanov, VV;Gruwel, MLH
通讯作者：
Gruwel, MLH
影响因子：
3.3
作者：
KRAIG, RP;NICHOLSON, C
通讯作者：
NICHOLSON, C