High Rates of O'Nyong Nyong and Chikungunya Virus Transmission in Coastal Kenya
High Rates of O'Nyong Nyong and Chikungunya Virus Transmission in Coastal Kenya
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DOI：
10.1371/journal.pntd.0003436
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发表时间：
2015-02-01
影响因子：
3.8
通讯作者：
King, Charles H.
中科院分区：
文献类型：
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作者：
LaBeaud, A. Desiree;Banda, Tamara;King, Charles H.
基孔肯雅病毒（CHIKV）和o'nyong nyong病毒（ONNV）是东非蚊子传播的甲型病毒，可引起急性发热性疾病和关节炎。本研究的目的是测量肯尼亚沿海地区CHIKV和ONNV的血清患病率，并将其与人口统计学和其他危险因素联系起来。方法对2009年从两个村庄（Milalani-Nganja和Vuga）招募的1,848名参与者进行了人口统计和暴露问卷调查。采用标准化的CHIKV IgG ELISA方案检测血清甲型病毒暴露情况，并采用斑块减少中和试验（PRNT）进行确认。使用逻辑回归模型确定与血清阳性相关的变量。采用加权K检验对50- 1000米范围内的甲型病毒阳性家庭进行全球聚类，并采用G*统计量和核密度图确定血清阳性率较高的地点。主要发现486例（26%）受试者IgG ELISA血清阳性。在443个PRNT确诊阳性样本中，25个样本（6%）为CHIKV+， 250个样本（56%）为ONNV+， 168个样本（38%）两种抗体的滴度都很高。年龄与血清阳性显著相关（OR为1.01 /年，95% ci为1.00-1.01）；然而，年轻人比老年人更有可能呈血清阳性。男性血清阳性的可能性较低(p
BackgroundChikungunya virus (CHIKV) and o'nyong nyong virus (ONNV) are mosquito-borne alpha-viruses endemic in East Africa that cause acute febrile illness and arthritis. The objectives of this study were to measure the seroprevalence of CHIKV and ONNV in coastal Kenya and link it to demographics and other risk factors.MethodologyDemographic and exposure questionnaires were administered to 1,848 participants recruited from two village clusters (Milalani-Nganja and Vuga) in 2009. Sera were tested for alphavirus exposure using standardized CHIKV IgG ELISA protocols and confirmed with plaque reduction neutralization tests (PRNT). Logistic regression models were used to determine the variables associated with seropositivity. Weighted K test for global clustering of houses with alphavirus positive participants was performed for distance ranges of 50-1,000 meters, and G* statistic and kernel density mapping were used to identify locations of higher seroprevalence.Principal Findings486 (26%) participants were seropositive by IgG ELISA. Of 443 PRNT confirmed positives, 25 samples (6%) were CHIKV+, 250 samples (56%) were ONNV+, and 168 samples (38%) had high titers for both. Age was significantly associated with seropositivity (OR 1.01 per year, 95% C.I. 1.00-1.01); however, younger adults were more likely to be seropositive than older adults. Males were less likely to be seropositive (p