Cytosolic heat-stress proteins Hsp17.7 class I and Hsp17.3 class II of tomato act as molecular chaperones in vivo
Cytosolic heat-stress proteins Hsp17.7 class I and Hsp17.3 class II of tomato act as molecular chaperones in vivo
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DOI：
10.1007/s004250000315
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发表时间：
2000-09-01
期刊：
影响因子：
4.3
通讯作者：
Forreiter, C
中科院分区：
文献类型：
--
作者：
Löw, D;Brändle, K;Forreiter, C
小分子热胁迫蛋白（Small heat-stress proteins，sHsps）是高等植物中含量最丰富的胁迫诱导蛋白，其成员多达20种。在细胞质中，可以区分出两种不同的类型。从秘鲁番茄（Lycopersicon peruvianum（L.）米尔，每个编码一个胞质sHsp亚家族，分析它们的转录本和蛋白质表达、基因组结构和伴侣活性。这两种类型都不存在控制条件下，但都出现在热应力和成熟的果实。发现II类转录本的表达在比I类转录本稍低的温度下诱导。使用类特异性抗体的蛋白质分析揭示了两种相应蛋白质的相同表达模式。在拟南芥（Arabodopsis thaliana（L.）嘿细胞培养表明，尽管它们的氨基酸序列不同，但这两类蛋白在体内作为伴侣蛋白是功能活性的，如它们在细胞环境中防止萤火虫荧光素酶热失活的能力所示。
Small heat-stress proteins (sHsps) are the most abundant stress-induced proteins with up to 20 different members in higher plants. In the cytoplasm, two different classes call be distinguished. Two cDNA clones from tomato Lycopersicon peruvianum (L.) Mill., each coding for one of the cytoplasmic sHsp subfamilies, were analyzed with respect to their transcript and protein expression, genome organization and chaperone activity. Neither type was present under control conditions but both appeared upon heat stress and in mature fruits. Expression of the class II transcript was found to be induced at slightly lower temperatures than the class I transcript. Protein analysis using class-specific antibodies revealed an identical expression pattern of both corresponding proteins. Transient expression in an Arabodopsis thaliana (L.) Heynh. cell culture showed that, despite the difference ill their amino acid sequence, both classes are functionally active as chaperones in vivo, as shown by their ability to prevent thermal inactivation of firefly luciferase in a cellular environment.