Unsuitability of using ribosomal RNA as loading control for Northern blot analyses related to the imbalance between messenger and ribosomal RNA content in rat mammary tumors

Unsuitability of using ribosomal RNA as loading control for Northern blot analyses related to the imbalance between messenger and ribosomal RNA content in rat mammary tumors
复制标题

DOI:
10.1006/abio.2000.4889
复制
发表时间:
2001-01-01
影响因子:
2.9
通讯作者:
Escrich, E
Escrich, E
中科院分区:
生物学4区
文献类型:
--
作者:
Solanas, M;Moral, R;Escrich, E

文献摘要

被引文献

相似文献

1. Rassow,J. 1999年蛋白质折叠和导入细胞器。在翻译后加工:一种实用的方法(Hames,BD和Higgins,SJ编辑),pp. 43-94.牛津大学出版社. 2. Schägger,H.,和von Jagow,G.(1991)用于分离酶活性形式的膜蛋白复合物的蓝色天然电泳。Anal. 199,223-231. 3. Dekker,PJT,Martin,F.,Maarse,AC,Bömer,U.,穆勒,H.,Guiard,B.,Meijer,M.,Rassow,J.,和Pfanner,N. Tim核心复合物定义了线粒体易位接触位点的数量,并且可以在不存在基质Hsp 70/Tim 44的情况下保持被捕的前蛋白。EMBO J. 16,5408-5419。4. Rassow,J.,Dekker,PJT,货车der Wilpe,S.,Meijer,M.,和Soll,J.(1999)线粒体内膜的前蛋白转位酶:功能和进化。J. Mol. 286,105-120中所述。5. Geissler,A.,Krimmer,T.,Schönfisch,B.,Meijer,M.,Rassow,J.(2000)酵母frataxin同系物Yfh 1 p的生物发生:Tim 44依赖性转移到mtHsp 70促进线粒体中新导入蛋白质的折叠。EUR. J. Biochem.267,3167-3180。
1. Rassow, J.(1999) Protein folding and import into organelles. In Post-translational Processing: A Practical Approach (Hames, BD, and Higgins, SJ, Eds.), pp. 43–94. IRL Press, Oxford. 2. Schägger, H., and von Jagow, G.(1991) Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form. Anal. Biochem. 199, 223–231. 3. Dekker, PJT, Martin, F., Maarse, AC, Bömer, U., Müller, H., Guiard, B., Meijer, M., Rassow, J., and Pfanner, N.(1997) The Tim core complex defines the number of mitochondrial translocation contact sites and can hold arrested preproteins in the absence of matrix Hsp70/Tim44. EMBO J. 16, 5408–5419. 4. Rassow, J., Dekker, PJT, van der Wilpe, S., Meijer, M., and Soll, J.(1999) The preprotein translocase of the mitochondrial inner membrane: Function and evolution. J. Mol. Biol. 286, 105–120. 5. Geissler, A., Krimmer, T., Schönfisch, B., Meijer, M., and Rassow, J.(2000) Biogenesis of the yeast frataxin homolog Yfh1p: Tim44-dependent transfer to mtHsp70 facilitates folding of newly imported proteins in mitochondria. Eur. J. Biochem. 267, 3167–3180.