Metabolism of glutathione in tumour cells as evidenced by 1H MRS
Metabolism of glutathione in tumour cells as evidenced by 1H MRS
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DOI：
10.1016/j.febslet.2007.01.025
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发表时间：
2007-02-20
期刊：
影响因子：
3.5
通讯作者：
Viti, V.
中科院分区：
文献类型：
--
作者：
Grande, S.;Luciani, A. M.;Viti, V.
IH MRS信号的谷胱甘肽和游离谷氨酸进行了检查，从培养的肿瘤细胞，即MCF-7从乳腺癌和TG 98从恶性胶质瘤的样品中，与信号强度有关的GSH代谢方面的目的。在不同细胞密度下收获的细胞的光谱表明GSH和Glu信号强度与细胞密度和增殖相关，并且它们的比率取决于γ-谷氨酰半胱氨酸合成酶的活性。通过对用抑制酶活性的丁噻鲁胺亚砜亚胺处理的细胞进行的实验证实了该假设。(c)2007年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
IH MRS signals of glutathione and of free glutamate were examined in samples from cultured tumour cells, namely MCF-7 from mammary carcinoma and TG98 from malignant glioma, with the aim of relating signal intensities to aspects of GSH metabolism. Spectra of cells harvested at different cell densities suggest that GSH and glu signal intensities are related to cell density and proliferation and their ratio is dependent on the activity of the gamma-glutamyl cysteine synthetase. The hypothesis is confirmed by experiments performed on cells treated with buthionine sulfoximine that inhibits the enzyme activity. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.