Utility of a microsatellite assay for identifying clonally related outbreak isolates of Aspergillus fumigatus
Utility of a microsatellite assay for identifying clonally related outbreak isolates of Aspergillus fumigatus
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DOI：
10.1016/j.mimet.2008.02.011
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发表时间：
2008-06-01
影响因子：
2.2
通讯作者：
Klaassen, Corne H. W.
中科院分区：
文献类型：
--
作者：
Balajee, S. Arunmozhi;de Valk, Hanneke A.;Klaassen, Corne H. W.
基于短串联重复序列（STRAf）的微卫星分析最近被描述为一种用于烟曲霉菌株指纹图谱的区分性高通量分析。然而，STRAf检测尚未测试其在暴发环境中的实用性，在暴发环境中区分克隆簇与遗传无关的基因型至关重要。在本研究中，采用一组流行病学关联的A。从6个不同的侵袭性曲霉病（IA）暴发获得的烟曲霉分离株，我们证明了STRAf检测可以是一个有价值的分子工具，以支持流行病学调查。我们还首次报告了在烟曲霉种群中检测到的微变异事件。(C)2008 Elsevier B.V.保留所有权利。
A microsatellite assay based on short tandem repeats (STRAf) has been recently described as a discriminatory, high throughput assay for fingerprinting Aspergillus fumigatus isolates. However, the STRAf assay has not been tested for its utility in outbreak settings where it is critical to distinguish clonal clusters from genetically unrelated genotypes. In the present study, employing a panel of epidemiologically linked A. fumigatus isolates obtained from 6 different outbreaks of invasive aspergillosis (IA), we demonstrate that the STRAf assay can be a valuable molecular tool to support epidemiological investigations. We also report for the first time the detection of microvariation events in the A.fumigatus population studied. (C) 2008 Elsevier B.V. All rights reserved.