Histogram clustering for rapid time-domain fluorescence lifetime image analysis.
Histogram clustering for rapid time-domain fluorescence lifetime image analysis.
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DOI:
10.1364/boe.427532
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发表时间:
2021-07-01
影响因子:
3.4
通讯作者:
Day-Uei Li D
中科院分区:
文献类型:
--
作者:
Li Y;Sapermsap N;Yu J;Tian J;Chen Y;Day-Uei Li D
We propose a histogram clustering (HC) method to accelerate fluorescence lifetime imaging (FLIM) analysis in pixel-wise and global fitting modes. The proposed method’s principle was demonstrated, and the combinations of HC with traditional FLIM analysis were explained. We assessed HC methods with both simulated and experimental datasets. The results reveal that HC not only increases analysis speed (up to 106 times) but also enhances lifetime estimation accuracy. Fast lifetime analysis strategies were suggested with execution times around or below 30 μs per histograms on MATLAB R2016a, 64-bit with the Intel Celeron CPU (2950M @ 2GHz).
影响因子:
4.6
作者:
García C;Losada A;Sacristán MA;Martínez-Leal JF;Galmarini CM;Lillo MP
通讯作者:
Lillo MP