A GDSL-type esterase/lipase gene, GELP77, is necessary for pollen dissociation and fertility in Arabidopsis.
A GDSL-type esterase/lipase gene, GELP77, is necessary for pollen dissociation and fertility in Arabidopsis.
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DOI：
10.1016/j.bbrc.2020.03.179
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发表时间：
2020-04
影响因子：
3.1
通讯作者：
D. Tsugama;K. Fujino;Shenkui Liu;T. Takano
中科院分区：
文献类型：
--
作者：
D. Tsugama;K. Fujino;Shenkui Liu;T. Takano
Pollen wall characteristics are dramatically changed during pollen maturation. Many genes have been identified as regulators of such changes in pollen wall characteristics, but mechanisms of such changes have not been completely understood. Here, a GDSL-type esterase/lipase gene,GELP77, is shown to regulate such changes inArabidopsisthaliana.GELP77-deficient (gelp77) plants exhibited male sterility, and this phenotype was suppressed by introduction of aGELP77genomic fragment. Mature pollen grains of wild-type Arabidopsis plants have an organized reticulate surface structure and are dissociated from each other. In contrast, pollen grains ofgelp77lacked such a structure and were shrunken and stuck to each other. Nuclei were not detectable ingelp77microspores at a putative uninucleate stage, suggesting thatGELP77is required as early as this stage. In plants that have theGELP77promoter-GELP77-GFPtransgene, the GELP77-GFP fusion protein was detected in microspores, tapetal cells and middle layer cells in anthers at post-meiotic stages, whereas not anthers at pre-meiotic stages. Analysis of amino acid sequences suggests thatGELP77is phylogenetically distant from the other 104 GDSL-type esterase/lipase genes in Arabidopsis and thatGELP77orthologs are present in various plant species. Together, these results indicate thatGELP77regulates pollen wall characteristics in Arabidopsis.
