MEMS-based handheld confocal microscope for in-vivo skin imaging.
MEMS-based handheld confocal microscope for in-vivo skin imaging.
复制标题
DOI：
10.1364/oe.18.003805
复制
发表时间：
2010-02-15
期刊：
影响因子：
3.8
通讯作者：
Mahadevan-Jansen A
中科院分区：
文献类型：
--
作者：
Arrasmith CL;Dickensheets DL;Mahadevan-Jansen A
本文介绍了一种用于皮肤显微镜的手持式激光扫描共聚焦显微镜。光束扫描是通过为皮科投影仪应用开发的电磁MEMS双轴向扫描器完成的，以每秒56帧的速度提供800 x600（SVGA）分辨率。该设计采用商用物镜，可选配半球前透镜，数值孔径范围为NA=0.35至NA=1.1，相应的对角视场范围为653 μm至216 μm。当NA=1.1，激光波长为830 nm时，测得轴向响应为1.14 μm半峰全宽，相应的10%-90%侧边响应为0.39 μm。提供了显示表皮和真皮特征（包括毛细血管血流）的图像示例。这些图像代表了使用MEMS双轴扫描镜进行组织成像所达到的最高分辨率和帧速率。
This paper describes a handheld laser scanning confocal microscope for skin microscopy. Beam scanning is accomplished with an electromagnetic MEMS bi-axial micromirror developed for pico projector applications, providing 800x600 (SVGA) resolution at 56 frames per second. The design uses commercial objective lenses with an optional hemisphere front lens, operating with a range of numerical aperture from NA=0.35 to NA=1.1 and corresponding diagonal field of view ranging from 653 μm to 216 μm. Using NA=1.1 and a laser wavelength of 830 nm we measured the axial response to be 1.14 μm full width at half maximum, with a corresponding 10%-90% lateral edge response of 0.39 μm. Image examples showing both epidermal and dermal features including capillary blood flow are provided. These images represent the highest resolution and frame rate yet achieved for tissue imaging with a MEMS bi-axial scan mirror.