A small RNA is functional in Escherichia fergusonii despite containing a large insertion.
A small RNA is functional in Escherichia fergusonii despite containing a large insertion.
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尽管含有大的插入片段，小RNA在弗格森埃希氏菌中仍具有功能。
DOI：
10.1099/mic.0.001099
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Raghavan,Rahul
中科院分区：
文献类型：
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作者：
Wright,AustinP;Dutcher,HAuguste;Butler,Brianna;Nice,TimothyJ;Raghavan,Rahul
细菌小rna （sRNAs）是基因表达的重要调控因子；然而，自然突变对sRNA功能的影响尚未得到广泛研究。在这里，我们发现sRNA MgrR在大肠杆菌和肠炎沙门氏菌的近亲——弗格森杆菌中包含一个独特的53 bp插入。插入是一个重复的基因外回文（REP）序列，可以阻断转录，但全长MgrR是由inE产生的。fergusonii，表明插入没有影响sRNA的产生。此外，尽管含有大的插入，sRNA似乎是功能性的，因为删除了mgrrmadee。对H2O2更敏感。MgrR在h2o2防御中的作用的分子细节尚未确定，但我们的研究结果表明，具有替代功能可以使sRNA保留在inE中。尽管它维持了一个巨大的，潜在的破坏性突变。
Bacterial small RNAs (sRNAs) are important regulators of gene expression; however, the impact of natural mutations on sRNA functions has not been studied extensively. Here we show that the sRNA MgrR contains a unique 53 bp insertion inEscherichia fergusonii, a close relative ofEscherichia coliandSalmonella enterica. The insertion is a repetitive extragenic palindromic (REP) sequence that could block transcription, but full-length MgrR is produced inE. fergusonii, showing that the insertion has not affected sRNA production. Additionally, despite containing the large insertion, the sRNA appears to be functional because deletion ofmgrRmadeE. fergusoniimore susceptible to H2O2. The molecular details of MgrR’s roles in H2O2defence are yet to be defined, but our results suggest that having an alternative function allowed the sRNA to be retained inE. fergusoniidespite it sustaining a large, potentially disruptive mutation.