Structural and functional aspects of three major glycoproteins of the human milk fat globule membrane.
Structural and functional aspects of three major glycoproteins of the human milk fat globule membrane.
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DOI：
10.1007/978-1-4615-1371-1_23
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发表时间：
2001
影响因子：
--
通讯作者：
Jerry A. Peterson;C. Scallan;Roberto L. Ceriani;M. Hamosh
中科院分区：
文献类型：
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作者：
Jerry A. Peterson;C. Scallan;Roberto L. Ceriani;M. Hamosh
MUC 1粘蛋白、乳粘附素和亲丁酸蛋白是人乳脂肪球膜的3种主要组分。粘蛋白抑制S-菌毛大肠杆菌与颊上皮细胞的结合，而乳粘蛋白可预防母乳喂养婴儿轮状病毒感染的症状。嗜丁酸蛋白被认为是人乳脂球（HMFG）膜的结构组分，并具有受体功能，但没有已知的抗感染活性。这些HMFG糖蛋白也存在于脱脂乳中，可能与磷脂胶束相关，而粘蛋白也以可溶形式存在。粘蛋白和乳粘附素在母乳喂养的婴儿的胃中抵抗消化，而亲丁酸蛋白则迅速降解。MUC 1粘蛋白是一种延伸的棒状结构，形成许多上皮细胞和乳汁膜表面糖萼的一部分，并可作为感染因子结合的诱饵。亲丁酸蛋白的胞外区段与IG超家族受体具有同源性，并且胞内结构域与发育调节蛋白具有同源性。Lactadherin是一种横向移动的细胞粘附分子，与整合素相互作用，并具有一种新的膜结合方式，涉及特异性结合磷脂酰丝氨酸。这些糖蛋白的结构和功能方面进行了讨论，就其在母乳喂养的婴儿母乳中的作用。
The MUC1 mucin, lactadherin, and butyrophilin are 3 major components of the human milk fat globule membrane. The mucin inhibits binding of S-fimbriatedEscherichia callto buccal epithelial cells, and lactadherin prevents symptomatic rotavirus infection in breast-fed infants. Butyrophilin has been suggested to be a structural component of the human milk fat globule (HMFG) membrane and to have receptor functions, but has no known anti-infective activity. These HMFG glycoproteins also are present in skimmed milk, possibly associated with phospholipid micelles, while mucin is also in a soluble form. Mucin and lactadherin resist digestion in the stomach of milk-fed infants, while butyrophilin is rapidly degraded. The MUC1 mucin is an extended rod-like structure forming part of the glycocalyx on the surface of many epithelial cells and membranes of milk, and may act as a decoy for binding of infective agents. The extracellular segment of butyrophilin has homology to Ig superfamily receptors and an intracellular domain with homology to developmentally regulated proteins. Lactadherin is a laterally mobile cell adhesion molecule that interacts with integrins and has a novel means of membrane-association involving specific binding to phosphatidylserine. The structural and functional aspects of these glyco-proteins are discussed with regard to their role in human milk for breast-fed infants.