Two-photon fluorescent turn-on probe for lipid rafts in live cell and tissue
Two-photon fluorescent turn-on probe for lipid rafts in live cell and tissue
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DOI：
10.1021/ja711391f
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发表时间：
2008-04-02
影响因子：
15
通讯作者：
Cho, Bong Rae
中科院分区：
文献类型：
--
作者：
Kim, Hwan Myung;Jeong, Byeong Ha;Cho, Bong Rae
我们报道了一种新的双光子荧光开启探针6-[（E）-3-oxo-1-dodecenyl]-2-[N-甲基-N-（羧甲基）氨基]萘（CL 2），它是专门设计用于观察活细胞和组织中的脂筏的。该探针在脂筏中发出比非脂筏结构域更亮的双光子激发荧光，并允许使用双光子显微镜在活组织中100-250 μ m深度处直接观察CA 1区域的活细胞和锥体神经元层中的脂筏。
We report a new two-photon fluorescence turn-on probe 6-[(E)-3-oxo-1-dodecenyl]-2-[N-methyl-N-(carboxymethyl)amino]naphthalene (CL2) that is designed specifically for visualizing lipid rafts in living cells and tissues. This probe emits much brighter two-photon excited fluorescence in lipid rafts than in non-raft domains and allows direct visualization of the lipid rafts in the live cells and pyramidal neuron layer of the CA1 region at a depth of 100-250 mu m in live tissues using two-photon microscopy.