Concomitant induction of the cell surface expression of Ia determinants and accessory cell function by a murine macrophage tumor cell line.
Concomitant induction of the cell surface expression of Ia determinants and accessory cell function by a murine macrophage tumor cell line.
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DOI：
10.1084/jem.155.2.629
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发表时间：
1982-02-01
期刊：
影响因子：
--
通讯作者：
Warner NL
中科院分区：
文献类型：
--
作者：
Walker EB;Lanier LL;Warner NL
这项研究表明，一个未知的可溶性因子产生的伴刀豆球蛋白A诱导的大鼠脾细胞悬液有能力诱导细胞表面H-2K和H-2D分子的表达增加和表达的I区基因产物的小鼠单核细胞-巨噬细胞系肿瘤，不是Ia阳性的情况下的因素。与诱导血清学定义的Ia特异性平行，Ia诱导的WEHI-3巨噬细胞肿瘤细胞能够提供辅助细胞功能，刺激以主要组织相容性复合物限制性、抗原依赖性方式激活的T-T杂交瘤产生IL-2。未诱导的Ia阴性WEHI-3肿瘤细胞在该测定系统中不引发可比较的应答。
This study demonstrates that an uncharacterized soluble factor produced in concanavalin A-induced rat spleen cell suspensions has the capacity to induce the increased expression of cell surface H-2K and H-2D molecules and the expression of I-region gene products on murine monocyte-macrophage lineage tumors that are not Ia positive in the absence of the factor. In parallel with induction of serologically defined Ia specificities, Ia-induced WEHI-3 macrophage tumor cells are capable of providing accessory cell function in stimulating IL-2 production by T-T hybridomas that are activated in a major histocompatibility complex-restricted, antigen-dependent fashion. The uninduced Ia-negative WEHI-3 tumor cells do not trigger a comparable response in this assay system.