Oxidative Stress-induced Posttranslational Modification of Proteins as a Target of Functional Food
Oxidative Stress-induced Posttranslational Modification of Proteins as a Target of Functional Food
复制标题
DOI：
10.1159/000212737
复制
发表时间：
2009-01-01
期刊：
影响因子：
--
通讯作者：
Yoshikawa, Toshikazu
中科院分区：
文献类型：
--
作者：
Naito, Yuji;Yoshikawa, Toshikazu
在与生活方式相关的疾病中，包括代谢综合征、动脉粥样硬化和癌症，氧化应激由几种标志物指示，其中包括脂质过氧化物、醛和硝基酪氨酸。我们假设，鉴定由于氧化应激而翻译后修饰的蛋白质将有助于更好地理解这些疾病中涉及变性和炎症的一些潜在分子机制。蛋白质组学是一种新兴的蛋白质及其修饰残基鉴定方法，在食品因子科学中的应用才刚刚开始。特别是，我们可以获得几种单克隆抗体来检测特异性氧化蛋白，这些单克隆抗体可用于免疫染色或免疫印迹分析。在这篇综述中，我们介绍了这些单克隆抗体在几种疾病中的应用，从中我们对这些疾病中与氧化应激相关的代谢和炎症机制有了新的认识。版权所有(C) 2009 S. Karger AG，巴塞尔
In lifestyle-related diseases including metabolic syndrome, atherosclerosis, and cancer, oxidative stress is indicated by several markers, among which are lipid peroxides, aldehydes, and nitrotyrosine. We hypothesized that identification of proteins that are posttranslationally modified due to oxidative stress would lead to a greater understanding of some of the potential molecular mechanisms involved in degeneration and inflammation in these disorders. Proteomics is an emerging method for identification of proteins and their modification residues, and its application to food factor science is just beginning. Especially, we can obtain several monoclonal antibodies to detect specifically oxidized proteins, which can be applied to analysis by immunostaining or immunoblot. In this review, we present the use of these monoclonal antibodies in several diseases, from which new insights have emerged into mechanisms of metabolism and inflammation in these disorders that are associated with oxidative stress. Copyright (C) 2009 S. Karger AG, Basel