Eluent Modifiers for the Liquid Chromatographic Separation of Carboxylic Acids Using Conductivity Detection
Eluent Modifiers for the Liquid Chromatographic Separation of Carboxylic Acids Using Conductivity Detection
复制标题
使用电导率检测进行羧酸液相色谱分离的洗脱液改性剂
DOI：
10.1021/ac00086a027
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发表时间：
1994
影响因子：
7.4
通讯作者：
J. Fritz
中科院分区：
文献类型：
--
作者：
John. Morris;J. Fritz
长期以来，人们一直认为，羧酸的色谱分离需要(A)使用相当大尺寸的凝胶型色谱柱和(B)酸性水洗脱液来抑制分析物的电离，从而产生尖锐的峰。目前的工作表明，这两个条件都不是必要的。在大孔树脂柱上，使用以水为主要洗脱剂的洗脱液，不添加酸，可获得良好的脂肪族羧酸分离。在水相流动相中加入醇，如正丁醇，对羧酸的色谱分离有很大的影响。酒精被认为覆盖在大孔聚苯乙烯-二乙烯基苯树脂的表面，并在流动相和固定相之间建立动态平衡
It has long been held that chromatographic separation of carboxylic acids requires (a) the use of a gel-type column of fairly large dimensions and (b) an acidic aqueous eluent to repress ionization of the analytes and thereby give sharp peaks. The present work shows that neither of these conditions is necessary. Excellent separations of aliphatic carboxylic acids were obtained on macroporous resin columns using a predominantly aqueous eluent with no added acid. Addition of an alcohol such as 1-butanol, to an aqueous mobile phase has a dramatic effect on the chromatographic separation of carboxylic acids. The alcohol is believed to coat the surface of a macroporous polystyrene-divinylbenzene resin and establish a dynamic equilibrium between the mobile and stationary phases