Immunocytochemical Analysis of DGKη in Cultured Cells Using a Monoclonal Antibody DhMab-4
Immunocytochemical Analysis of DGKη in Cultured Cells Using a Monoclonal Antibody DhMab-4
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使用单克隆抗体 DhMab-4 对培养细胞中的 DGKη 进行免疫细胞化学分析
DOI：
10.1089/mab.2021.0034
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发表时间：
2021
影响因子：
--
通讯作者：
Goto Kaoru
中科院分区：
文献类型：
--
作者：
Nakano Tomoyuki;Tanaka Toshiaki;Sakane Fumio;Kaneko Mika K.;Kato Yukinari;Goto Kaoru
甘油二酯激酶（DGK）是一种将甘油二酯（DG）转化为磷脂酸（PA）的脂质激酶。由于DG和PA都是细胞内的第二信使分子，因此DGK在平衡这两种信号通路中起着关键作用。在DGK家族中，DGKη被分类为II型DGK。据报道，DGKη通过哺乳动物组织和细胞普遍表达。先前使用cDNA转染方法的研究报道了DGKη在培养的人细胞中的细胞质定位。然而，天然蛋白质的亚细胞定位仍然是未知的。最近，我们建立了人DGKη特异性单克隆抗体DhMab-4。在这项研究中，我们研究了DGKη的天然蛋白的亚细胞定位使用DhMab-4在人培养细胞中的免疫细胞化学。
Diacylglycerol kinase (DGK) is a lipid kinase that converts diacylglycerol (DG) to phosphatidic acid (PA). Since both DG and PA serve as intracellular second messenger molecules, DGK plays a pivotal role in balancing these two signaling pathways. Of the DGK family, DGKη is classified as a type II DGK. Reportedly, DGKη is expressed ubiquitously through mammalian tissues and cells. Previous studies using cDNA transfection methods reported cytoplasmic localization of DGKη in cultured human cells. However, subcellular localization of native protein is still unknown. Recently, we established a human DGKη-specific monoclonal antibody, DhMab-4. In this study, we examined subcellular localization of native protein of DGKη using DhMab-4 by immunocytochemistry in human cultured cells.