Detection and molecular characterization of Peste des Petits Ruminants virus from outbreaks in Burundi, December 2017-January 2018
Detection and molecular characterization of Peste des Petits Ruminants virus from outbreaks in Burundi, December 2017-January 2018
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DOI：
10.1111/tbed.13255
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发表时间：
2019-09-01
影响因子：
4.3
通讯作者：
Bodjo, Sanne Charles
中科院分区：
文献类型：
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作者：
Niyokwishimira, Alfred;Baziki, Jean de D.;Bodjo, Sanne Charles
2017年12月，布隆迪（东非）出现小反刍兽疫（PPR），并迅速蔓延至该国5个省（基特加、基隆多、姆瓦罗、穆拉姆维亚和卡鲁齐），造成严重疾病，仅基特加省就有4,000多只山羊死亡。布隆迪政府兽医服务部门于2017年12月进行了初步疫情调查，并收集了样本进行实验室确认。采用兰州兽医研究所提供的竞争性酶联免疫吸附试验（cELISA：中国专利号ZL201210278970.9）检测112份血清，结果显示约37.5%的阳性样品。在先前未报告PPR感染和疫苗接种的动物群体中，这种高水平的PPR阳性血清表明动物暴露于PPRV。随后在2018年1月，在非洲联盟-泛非动物资源局（AU-IBAR），AU-PANVAC和东非共同体（EAC）的联合调查使命之后，在非洲联盟-泛非兽医疫苗中心（AU-PANVAC）实验室进行的实验室测试证实了布隆迪存在PPR。通过常规RT-PCR检测的样品表明存在PPR病毒（PPRV）。还进行了病毒的确证性分离。系统发育分析显示，该病毒属于谱系III，与2011年肯尼亚和2012年乌干达的PPRV分离株关系密切。对2017年12月至2018年2月期间布隆迪爆发PPR的可能解释进行了介绍。
In December 2017, Peste des Petits Ruminants (PPR) emerged in Burundi (East Africa) and rapidly spread to five provinces (Gitega, Kirundo, Mwaro, Muramvya and Karuzi) in the country, causing severe disease and killing more than 4,000 goats in the province of Gitega alone. An initial outbreak investigation was conducted in December 2017 by the Burundi Government Veterinary Services and samples were collected for laboratory confirmation. A competitive Enzyme Linked Immuno-Sorbent Assay (cELISA: Chinese Patent No. ZL201210278970.9) supplied by the Lanzhou Veterinary Research Institute was used to test 112 sera and results showed around 37.5% positive samples. This high level of PPR positive sera in an animal population where PPR infection and vaccination had not been previously reported indicated the exposure of the animals to PPRV. Subsequently in January 2018, the laboratory tests conducted at the African Union-Pan African Veterinary Vaccine Centre (AU-PANVAC) laboratories following a joint investigative mission by the African Union-Interafrican Bureau for Animal Resources (AU-IBAR), AU-PANVAC and the East African Community (EAC) confirmed the presence of PPR in Burundi. Samples tested by conventional RT-PCR indicated the presence of the PPR virus (PPRV). Confirmatory isolation of the virus was also performed. Phylogenetic analysis revealed that the virus belongs to lineage III and shows a close relationship with PPRV isolates from Kenya in 2011 and Uganda in 2012. A possible explanation for the outbreaks of PPR in Burundi between December 2017 and February 2018 is presented.