Degradation of ferric EDTA by Burkhol cepacia
Degradation of ferric EDTA by Burkhol cepacia
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Burkhol cepacia 降解 EDTA 铁
DOI：
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发表时间：
2003
影响因子：
3
通讯作者：
Szu
中科院分区：
文献类型：
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作者：
H. Fang;Shin;Szu
EDTA是本研究的目标化合物，来自二级生物处理单元的出水，可以被特殊的微生物生物降解。到目前为止，有三种微生物-土壤杆菌，革兰氏阴性BNCI和DSM 9103-可以降解EDTA，并发表在文献中。我们已经成功地分离出一种细菌菌株，可以降解EDTA。经鉴定，该菌为洋葱伯克霍菌（Burkhol cepacia），椭圆形，长5-15 μm，需氧。培养基中碳源为1000 mg/L的EDTA铁，氮源为750 mg/L的（NH 4）2SO 4+（NH 2）2CO，磷源为1000 mg/L的KH 2 PO 4，以及铁、镁等矿质因子。在pH7.0、30°C和150 rpm的摇床上培养15 d，该微生物的平均比生长速率为0.135 d-1，这表明Fe-EDTA和Cu-EDTA的降解效率分别为90%和75%。
EDTA, the target compound of this study from the effluent of secondary biotreatment units, can be biodegraded by special microorganisms. So far, there are three species of microorganisms—Agrobacterium, Gram-negative BNCI, and DSM9103—that can degrade EDTA and are published in the literature. We have successfully isolated a bacterial strain that can degrade EDTA. It was identified as Burkhol cepacia, an aerobic species, elliptically shaped with a length of 5–15 μm. The growth medium contains 1000 mg/L of ferric-EDTA as carbon source, 750 mg/L of (NH4)2SO4+(NH2)2CO as nitrogen source, and 1000 mg/L of KH2PO4 as phosphorus source, and mineral factors such as Fe and Mg. Incubated at pH, 7.0, 30°C, and 150 rpm on a shaker for 15 d, the average specific growth rate of this microbe is 0.135 d−1, which shows that the respective degradation efficiency of Fe-EDTA and Cu-EDTA is 90 and 75% individually.