The endonuclease Ankle1 requires its LEM and GIY-YIG motifs for DNA cleavage in vivo.
The endonuclease Ankle1 requires its LEM and GIY-YIG motifs for DNA cleavage in vivo.
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DOI：
10.1242/jcs.098392
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发表时间：
2012-02-15
影响因子：
4
通讯作者：
Foisner R
中科院分区：
文献类型：
--
作者：
Brachner A;Braun J;Ghodgaonkar M;Castor D;Zlopasa L;Ehrlich V;Jiricny J;Gotzmann J;Knasmüller S;Foisner R
层相关多肽Emerin， MAN1 - (LEM)结构域定义了一组核蛋白，通过LEM基序与保守的DNA交联蛋白BAF （Barrier-to-Auto-Integration factor）相互作用结合染色质。在这里，我们描述了一个新的LEM蛋白，在数据库中注释为“ANKLE1”（ANKLE1）。我们发现Ankle1在后生动物中是保守的，并且包含一个独特的c端GIY-YIG基序，该基序在体外和体内都具有内切酶活性。在哺乳动物中，Ankle1主要在造血组织中表达。虽然大多数LEM蛋白是核膜的组成部分，但异位Ankle1穿梭于细胞质和细胞核之间，并且在核质中富集的Ankle1诱导DNA切割和DNA损伤反应。这种活性既需要催化c端GIY-YIG结构域，也需要通过BAF结合染色质的LEM基序。因此，Ankle1代表了一种在高等真核生物中具有GIY-YIG型内切酶活性的新型lem蛋白。
The Lamina-associated polypeptide, Emerin, MAN1 - (LEM) domain defines a group of nuclear proteins, which bind chromatin through interaction of the LEM motif with the conserved DNA cross-linking protein, Barrier-to-Auto-Integration factor (BAF). Here, we describe a novel LEM protein, annotated in databases as “Ankyrin and LEM domain containing protein 1” (ANKLE1). We show that Ankle1 is conserved in metazoans and contains a unique C-terminal GIY-YIG motif that confers endonuclease activity in vitro and in vivo. In mammals, Ankle1 is predominantly expressed in hematopoietic tissues. While most characterized LEM proteins are components of the inner nuclear membrane, ectopic Ankle1 shuttles between cytoplasm and nucleus, and Ankle1 enriched in the nucleoplasm induces DNA cleavage and DNA damage response. This activity requires both the catalytic C-terminal GIY-YIG domain and the LEM motif, which binds chromatin via BAF. Hence, Ankle1 represents a novel LEM-protein with a GIY-YIG type endonuclease activity in higher eukaryotes.
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影响因子：
64.5
作者：
Fekairi S;Scaglione S;Chahwan C;Taylor ER;Tissier A;Coulon S;Dong MQ;Ruse C;Yates JR 3rd;Russell P;Fuchs RP;McGowan CH;Gaillard PHL
通讯作者：
Gaillard PHL
影响因子：
11.4
作者：
Cai, ML;Huang, Y;Clore, GM
通讯作者：
Clore, GM
影响因子：
4
作者：
Dechat, T;Gajewski, A;Foisner, R
通讯作者：
Foisner, R
影响因子：
4.8
作者：
Holaska, JM;Lee, KK;Wilson, KL
通讯作者：
Wilson, KL
DOI：
10.1073/pnas.88.17.7719
发表时间：
1991-09-01
影响因子：
11.1
作者：
BELLPEDERSEN, D;QUIRK, SM;BELFORT, M
通讯作者：
BELFORT, M