Thermal conductivity of cryoprotective agents loaded with nanoparticles, with application to recovery of preserved tissues and organs from cryogenic storage
Thermal conductivity of cryoprotective agents loaded with nanoparticles, with application to recovery of preserved tissues and organs from cryogenic storage
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DOI：
10.1371/journal.pone.0238941
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发表时间：
2020-09-17
期刊：
影响因子：
3.7
通讯作者：
Rabin, Yoed
中科院分区：
文献类型：
--
作者：
Ehrlich, Lili E.;Gao, Zhe;Rabin, Yoed
本研究的目的是提供基于 CPA 的纳米流体的热导率数据，以利于玻璃化冷冻保存的分析。热导率测量是在低温宏观显微镜的实验平台上使用热线技术进行的，将测量结果与观察到的物理效应（例如结晶和破裂）相关联。本研究中测试的材料包括 CPA 混合物 M22、VS55、DP6 和 DP6+蔗糖。本研究中的纳米流体包括上述 CPA 混合物作为基础溶液，与氧化铁纳米粒子 (IONP) 或二氧化硅涂层氧化铁纳米粒子 (sIONP) 混合。这项研究的结果表明，在任何测试的 CPA 混合物中添加 sIONP 都不会显着影响其导热性、玻璃化趋势，或者相反，其形成复温相结晶 (RPC) 的趋势。尽管 sIONP 溶液具有固有的不透明性，但在仔细控制的复温条件下，在没有射频激活的情况下，在 DP6+sIONP 复温开始时，用冷冻宏观镜观察到骨折。使用射频加热加速复温并统一温度分布可能会防止断裂和 RPC。然而，本研究中并未激活 sIONP，因为射频加热机制会干扰热导率测量。在 DP6 中添加 IONP 似乎会阻碍 CPA 玻璃化的趋势，这是一种有害的影响。但未涂覆的纳米颗粒溶液不太可能用于实际应用。
The objective of this study is to provide thermal conductivity data for CPA-based nanofluids for the benefit of the analyses of cryopreservation by vitrification. Thermal conductivity measurements were conducted using a hot-wire technique on an experimentation platform of the cryomacroscope, to correlate measurements with observed physical effects such as crystallization and fracturing. Tested materials in this study include the CPA cocktails M22, VS55, DP6, and DP6+sucrose. Nanofluids in this study include the above CPA cocktails as base solutions, when mixed with either iron-oxide nanoparticles (IONP) or silica-coated iron-oxide nanoparticles (sIONP). Results of this study demonstrated the addition of sIONP to any of the CPA cocktails tested did not significantly affect its thermal conductivity, its tendency to vitrify or, conversely, its tendency to form rewarming phase crystallization (RPC). Fractures were observed with cryomacroscopy at the onset of rewarming for DP6+sIONP under carefully controlled rewarming conditions without RF activation, despite the inherent opacity of the sIONP solutions. It is likely that using RF heating in order to accelerate rewarming while unifying the temperature distribution would prevent fracture and RPC. However, sIONP were not activated in this study, as the RF heating mechanism would interfere with thermal conductivity measurements. The addition of IONP to DP6 appears to hinder the tendency of the CPA to vitrify, which is a detrimental effect. But it is unlikely that uncoated nanoparticle solutions will be used in practical applications.