Ferroptosis is involved in the anti-tumor effect of lycorine in renal cell carcinoma cells.
Ferroptosis is involved in the anti-tumor effect of lycorine in renal cell carcinoma cells.
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DOI：
10.3892/ol.2021.13042
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发表时间：
2021-11
期刊：
影响因子：
2.9
通讯作者：
Wang L
中科院分区：
文献类型：
--
作者：
Du Y;Zhao HC;Zhu HC;Jin Y;Wang L
肾细胞癌是泌尿生殖系统最常见的恶性肿瘤之一。研究表明石蒜碱具有良好的抗癌活性，副作用小。石蒜碱对肾癌细胞增殖的影响及其抗肿瘤机制尚未完全阐明。培养人肾癌细胞系786-O、A498和Caki-1，用不同浓度的石蒜碱或铁凋亡抑制剂ferrostatin-1处理。使用细胞活力和集落形成测定来测量细胞增殖。分析了5-、12-和15-HETE羟基二十碳四烯酸（HETE）和MDA水平，以及还原型谷胱甘肽与氧化型谷胱甘肽（GHS/GSSG）的比值。免疫印迹法检测谷胱甘肽过氧化物酶4（GPX 4）和酰基辅酶A合成酶长链家族成员4（ACSL 4）的表达。透射电子显微镜观察与铁下垂相关的形态学特征。石蒜碱可抑制肾癌细胞的增殖。石蒜碱处理后，RCC细胞中GPX 4的表达水平降低，而ACSL 4的表达水平升高。石蒜碱可诱导肾癌细胞5-HETE、12-HETE、15-HETE和MDA的表达，并降低GSH/GSSG比值。此外，ferostatin-1可防止石蒜碱诱导的RCC细胞铁凋亡。
Renal cell carcinoma (RCC) is a most common malignant tumor in the genitourinary system. Studies have shown that Lycorine has promising anticancer activities with minor side effects. However, the effect of lycorine on the proliferation of RCC cells and its underlying anti-tumor mechanism have not yet been fully elucidated. The human renal cancer cell lines 786-O, A498 and Caki-1 were cultured and treated with different concentrations of lycorine or ferrostatin-1, a ferroptosis inhibitor. Cell viability and colony formation assays were used to measure cell proliferation. The 5-, 12- and 15-HETE hydroxyeicosatetraenoic acid (HETE) and MDA levels, as well as the reduced to oxidized glutathione (GHS/GSSG) ratio, were analyzed. Western blot analysis was used to detect the expression of glutathione peroxidase 4 (GPX4) and acyl-CoA synthetase long chain family member 4 (ACSL4), which are key markers of ferroptosis. Transmission electron microscopy was used to observe the morphological features associated with ferroptosis. Lycorine was found to inhibit the proliferation of RCC cells. After lycorine treatment, the expression levels of GPX4 in RCC cells decreased, whereas those of ACSL4 increased. Lycorine induced the expression of 5-HETE, 12-HETE, 15-HETE and MDA in RCC cells, and reduced the GSH/GSSG ratio. In addition, ferrostatin-1 could prevent lycorine-induced ferroptosis in RCC cells.
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影响因子：
4.7
作者：
Argiris A;Harrington KJ;Tahara M;Schulten J;Chomette P;Ferreira Castro A;Licitra L
通讯作者：
Licitra L
Lycorine 下调 HMGB1 以抑制自噬并增强多发性骨髓瘤中硼替佐米的活性
影响因子：
12.4
作者：
Roy M;Liang L;Xiao X;Peng Y;Luo Y;Zhou W;Zhang J;Qiu L;Zhang S;Liu F;Ye M;Zhou W;Liu J
通讯作者：
Liu J
石蒜碱：抗癌药物发现的潜在天然先导物质
DOI：
10.1016/j.biopha.2018.07.147
发表时间：
2018-11
期刊：
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
影响因子：
--
作者：
Roy M;Liang L;Xiao X;Feng P;Ye M;Liu J
通讯作者：
Liu J
影响因子：
7.3
作者：
Lamoral-Theys D;Andolfi A;Van Goietsenoven G;Cimmino A;Le Calvé B;Wauthoz N;Mégalizzi V;Gras T;Bruyère C;Dubois J;Mathieu V;Kornienko A;Kiss R;Evidente A
通讯作者：
Evidente A
影响因子：
4.2
作者：
Cheng, Jing;Fan, Yan-Qin;Chen, Qian-Xue
通讯作者：
Chen, Qian-Xue