Genome-wide and expression-profiling analyses of the cytochromeP450genes inBactrocera dorsalis(Hendel) and screening of candidateP450genes associated with malathion resistance
Genome-wide and expression-profiling analyses of the cytochromeP450genes inBactrocera dorsalis(Hendel) and screening of candidateP450genes associated with malathion resistance
复制标题
橘小实蝇（Hendel）细胞色素P450基因的全基因组和表达谱分析以及与马拉硫磷抗性相关的候选P450基因的筛选
DOI：
10.1002/ps.5891
复制
发表时间：
2020-06-13
影响因子：
4.1
通讯作者：
Wang, Jin-Jun
中科院分区：
文献类型：
--
作者：
Jing, Tian-Xing;Wang, Di-Fei;Wang, Jin-Jun
背景桔小实蝇Bactrocera dorsalis(Hendel)是一种世界性的农业害虫，对杀虫剂的抗药性是防治成功的主要障碍。细胞色素P450s(P450s)是与抗药性相关的主要代谢酶。B的基因组。Dolsalis最近被测序，使得能够对这种害虫的P450基因(P450)进行完整的全基因组分析，并分析这些基因与杀虫剂抗性之间的相关性。结果B组共鉴定出101个P450。并将其划分为4个科、25个科和57个亚科。定量逆转录聚合酶链式反应结果显示，这些基因大多在成人(46例)和代谢组织中高表达，包括脂肪体(63例)、中肠(61例)和马尔菲氏小管(66例)。在马拉硫磷抗性品系中，与敏感品系相比，分别有13个和9个基因显著上调和下调，这些基因被筛选为与马拉硫磷抗性相关的候选基因。结论本研究为了解P450sinB的进化和潜在功能提供了有用的信息。为进一步研究P450与马拉硫磷抗性之间的关系奠定了基础。多萨利斯。
BACKGROUND Bactrocera dorsalis(Hendel) is a notorious agricultural pest worldwide, and its resistance to insecticides is a major obstacle in successful control. Cytochrome P450s (P450s) are major metabolic enzymes associated with insecticide resistance. The genome ofB. dorsaliswas sequenced recently, allowing an integrated genome-wide analysis of P450 genes (P450s) and the analysis of correlations between these genes and insecticide resistance in this pest. RESULTS Totally, 101P450swere identified in theB. dorsalisgenome and classified into four clans, 25 families and 57 subfamilies. Quantitative reverse transcription polymerase chain reaction results showed that most of these genes were highly expressed in adults (46) and in metabolic tissues, including the fatbody (63), midgut (61) and Malphagian tubules (66). In a malathion-resistant strain, 13 and 9 genes were significantly upregulated and downregulated, respectively, compared with a susceptible strain, and these genes were screened as candidate genes associated with malathion resistance. CONCLUSION This study provides useful information for understanding the evolution and potential functions ofP450sinB. dorsalis, and the results lay the foundation for further studies on the correlations betweenP450sand malathion resistance inB. dorsalis.