The signalling peptide EPFL9 is a positive regulator of stomatal development
The signalling peptide EPFL9 is a positive regulator of stomatal development
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DOI：
10.1111/j.1469-8137.2010.03200.x
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发表时间：
2010-01-01
期刊：
影响因子：
9.4
通讯作者：
Gray, Julie E.
中科院分区：
文献类型：
--
作者：
Hunt, Lee;Bailey, Karen J.;Gray, Julie E.
P>假定的分泌肽表皮模式因子 1 (EPF1) 和 EPF2 在拟南芥叶片发育早期充当气孔聚集和密度的负调节因子。在这里，我们研究了与 EPF1 和气孔密度和分布 1 (SDD1) 共表达的相关肽基因表皮模式因子 9 (EPFL9) 是否也在控制气孔发育中发挥作用。组成型过表达 EPFL9 的气孔密度和聚类增加，而 EPFL9 表达减少的表达则显示气孔密度降低，但没有聚类，证实 EPFL9 是气孔发育的调节因子。遗传分析与 EPFL9 独立于 EPF1 控制气孔聚集、独立于 EPF2 调节气孔密度以及独立于 SDD1 控制气孔聚集和密度一致。这些发现表明，至少三种分泌肽独立调节气孔发育。令人惊讶的是，EPFL9 的作用是增加而不是减少气孔密度和聚集。然而，与 EPF1 和 EPF2 一样，EPFL9 不太可能成为 SDD1 蛋白水解的底物。
P>The putative secretory peptides epidermal patterning factor 1 (EPF1) and EPF2 act as negative regulators of stomatal clustering and density early in Arabidopsis leaf development.Here, we investigated whether the related peptide gene epidermal patterning factor-like 9 (EPFL9), which is coexpressed with EPF1 and stomatal density and distribution 1 (SDD1), also plays a role in controlling stomatal development.Plants manipulated to constitutively overexpress EPFL9 showed increased stomatal density and clustering, and those manipulated to have reduced EPFL9 expression showed reduced stomatal density with no clustering, confirming that EPFL9 is a regulator of stomatal development. Genetic analysis was consistent with EPFL9 acting independently of EPF1 to control stomatal clustering, independently of EPF2 to regulate stomatal density, and independently of SDD1 to control both stomatal clustering and density.These findings demonstrate that at least three secretory peptides independently regulate stomatal development. Surprisingly, EPFL9 acts to increase, rather than decrease, stomatal density and clustering. However, in common with EPF1 and EPF2, EPFL9 is unlikely to be a substrate for proteolysis by SDD1.