The innervation of synovium of human osteoarthritic joints in comparison with normal rat and sheep synovium
The innervation of synovium of human osteoarthritic joints in comparison with normal rat and sheep synovium
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DOI：
10.1016/j.joca.2013.06.018
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发表时间：
2013-09-01
影响因子：
7
通讯作者：
Schaible, H. -G.
中科院分区：
文献类型：
--
作者：
Eitner, A.;Pester, J.;Schaible, H. -G.
目的：研究膝关节骨关节炎（OA）是否与滑膜层神经支配模式的变化有关。设计：在大鼠和绵羊正常膝关节的滑膜组织中，我们使用透射电子显微镜和免疫组织化学研究了血管和神经纤维的存在。滑膜材料也从接受全膝关节置换手术的患者身上获得。检查该材料的炎症变化，并评估血管和神经纤维的存在。结果：大鼠和绵羊正常膝关节髌旁区域的滑膜表现出致密的毛细血管和神经元网络。它由含有感觉纤维和酪氨酸羟化酶阳性交感神经纤维的降钙素基因相关肽进入。膝关节骨关节炎患者的滑膜材料表现出不同程度的炎症。没有炎症的滑膜材料表现出与大鼠和绵羊的正常膝关节相似的血管和神经元网络。然而，在发生炎症变化的滑膜中，我们发现靠近滑膜内层的深度范围内的神经纤维显着减少，具体取决于炎症程度，而较深的区域受影响较小。结论：OA关节滑膜的炎症变化与正常滑膜中存在的毛细血管和神经元网络的大规模破坏有关。由于感觉纤维的消失，骨关节炎疼痛不太可能直接在滑膜中引发。正常神经支配的血管化的丧失可能对滑膜的生理功能产生多种后果。 (C) 2013 年国际骨关节炎研究协会。由爱思唯尔有限公司出版。保留所有权利。
Objective: To study whether osteoarthritis (OA) in the knee is associated with a change of the innervation pattern in the synovial layer.Design: In synovial tissue from the normal knee joint of rat and sheep we studied the presence of vessels and of nerve fibres using transmission electron microscopy and immunohistochemistry. Synovial material was also obtained from patients who underwent total knee replacement surgery. This material was examined for inflammatory changes, and the presence of vessels and nerve fibres was assessed.Results: The synovium in the parapatellar region of the normal knee joint of rat and sheep exhibited a dense capillary and neuronal network. It was entered by calcitonin gene-related peptide containing sensory fibres and tyrosine hydroxylase-positive sympathetic nerve fibres. Synovial material from patients with knee OA exhibited different degrees of inflammation. Synovial material without inflammation exhibited a similar vascular and neuronal network as the normal knee joint from rat and sheep. However, in synovium with inflammatory changes we found a significant decrease of nerve fibres in depth ranges close to the synovial lining layer depending on the degree of inflammation whereas deeper regions were less affected.Conclusions: Inflammatory changes in the synovium of OA joints are associated with a massive destruction of the capillary and neuronal network which is present in normal synovium. Due to the disappearance of the sensory fibres it is unlikely that OA pain is initiated directly in the synovium. The loss of normally innervated vascularisation may have multiple consequences for the physiological functions of the synovium. (C) 2013 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.