Tumor specific ultrasound enhanced gene transfer in vivo with novel liposomal bubbles
Tumor specific ultrasound enhanced gene transfer in vivo with novel liposomal bubbles
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DOI：
10.1016/j.jconrel.2007.08.025
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发表时间：
2008-01-22
影响因子：
10.8
通讯作者：
Maruyama, Kazuo
中科院分区：
文献类型：
--
作者：
Suzaki, Ryo;Takizawa, Tornoko;Maruyama, Kazuo
泡脂质体（捕获超声成像气体的脂质体）可以构成一种独特的系统，用于在体外将各种分子有效地递送到哺乳动物细胞中。在本研究中，气泡脂质体与阳离子脂质(CL)-DNA复合物在体内作为肿瘤基因传递载体进行了比较。气泡脂质体的基因传递取决于所施加的超声强度。转染效率在0.7 W/cm(2)超声强度下趋于稳定。气泡脂质体可以有效地将基因转移到培养细胞中，即使细胞暴露在超声波下仅1 s。此外，气泡脂质体比CL-DNA复合物更能有效地将荧光素酶基因引入小鼠腹水肿瘤细胞和实体瘤组织。我们认为气泡脂质体与超声结合是一种微创的肿瘤特异性基因体内转移方法。(C) 2007 Elsevier B.V.版权所有
Bubble liposomes (liposomes which entrap an ultrasound imaging gas) may constitute a unique system for delivering various molecules efficiently into mammalian cells in vitro. In this study, Bubble liposomes were compared with cationic lipid (CL)-DNA complexes as potential gene delivery carriers into tumor in vivo. The delivery of genes by Bubble liposomes depended on the intensity of the applied ultrasound. Transfection efficiency plateaued at 0.7 W/cm(2) ultrasound intensity. Bubble liposomes efficiently transferred genes into cultured cells even when the cells were exposed to ultrasound for only 1 s. In addition, Bubble liposomes could introduce the luciferase gene more effectively than CL-DNA complexes into mouse ascites tumor cells and solid tumor tissue. We conclude that the combination of Bubble liposomes and ultrasound is a minimally-invasive and tumor specific gene transfer method in vivo. (C) 2007 Elsevier B.V. All rights reserved.