Identification of spontaneous mutations within the long-range limb-specific Sonic hedgehog enhancer (ZRS) that alter Sonic hedgehog expression in the chicken limb mutants oligozeugodactyly and silkie breed.
Identification of spontaneous mutations within the long-range limb-specific Sonic hedgehog enhancer (ZRS) that alter Sonic hedgehog expression in the chicken limb mutants oligozeugodactyly and silkie breed.
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DOI：
10.1002/dvdy.22634
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发表时间：
2011-05
影响因子：
2.5
通讯作者：
Fallon, John F.
中科院分区：
文献类型：
--
作者：
Maas, Sarah A.;Suzuki, Takayuki;Fallon, John F.
关键词：
limb developmentlimb bud prepatternSonic hedgehog (SHH), LMBR1oligozeugodactyly (ozd)zone of polarizing activity (ZPA)cis-enhancerreporter analysisZPA regulatory sequence (ZRS)mammals-fishes-conserved-sequence-1 (MFCS-1)Acheiropodia (ACHP)preaxial polydactyly (PPD)Dorking Breed chickenSilkie Breed chicken
进化上保守的非编码~800碱基对极化活性区（ZPA）调控序列（ZRS）控制后肢Shh的表达。我们报告说，鸡突变oligozeugodeptide（厄兹德），缺乏肢体Shh表达，有一个大的ZRS内删除。此外，发育异位前肢Shh表达的前轴多指、丝羽乌骨鸡在ZRS内具有单个碱基对变化。使用体内报告基因测定来检查鸡肢中的增强子功能，我们证明野生型ZRS驱动野生型和厄兹德肢的ZPA中的β-半乳糖苷酶报告基因表达。在野生型肢芽中，丝羽ZRS在后Shh结构域和前Shh结构域两者中驱动β-半乳糖苷酶。这些结果支持了ZRS在鸡模型系统中整合了正向和负向预模式化调控输入的假设，并证明了鸡肢体作为基因调控研究的有效遗传系统的实用性。
The evolutionarily conserved, non-coding ~800 base-pair zone of polarizing activity (ZPA) regulatory sequence (ZRS) controls Shh expression in the posterior limb. We report that the chicken mutant oligozeugodactly (ozd), which lacks limb Shh expression, has a large deletion within the ZRS. Furthermore, the preaxial polydactylous, Silkie Breed chicken, which develops ectopic anterior limb Shh expression, has a single base-pair change within the ZRS. Using an in vivo reporter assay to examine enhancer function in the chick limb, we demonstrate that the wild-type ZRS drives β-galactosidase reporter expression in the ZPA of both wild-type and ozd limbs. The Silkie ZRS drives β-galactosidase in both posterior and anterior Shh domains in wild-type limb buds. These results support the hypothesis that the ZRS integrates positive and negative prepatterned regulatory inputs in the chicken model system and demonstrate the utility of the chicken limb as an efficient genetic system for gene regulatory studies.
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影响因子：
9.2
作者：
LOPEZMARTINEZ, A;CHANG, DT;FALLON, JF
通讯作者：
FALLON, JF
影响因子：
2.7
作者：
Chiang, C;Litingtung, Y;Fallon, JF
通讯作者：
Fallon, JF
DOI：
10.1073/pnas.112212199
发表时间：
2002-05-28
影响因子：
11.1
作者：
Lettice, LA;Horikoshi, T;Noji, S
通讯作者：
Noji, S
影响因子：
2.6
作者：
Liu, AM;Joyner, AL;Turnbull, DH
通讯作者：
Turnbull, DH
影响因子：
10.5
作者：
MASUYA, H;SAGAI, T;SHIROISHI, T
通讯作者：
SHIROISHI, T