Developmental regulation of spine motility in the mammalian central nervous system
Developmental regulation of spine motility in the mammalian central nervous system
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DOI：
10.1073/pnas.96.23.13438
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发表时间：
1999-11-09
影响因子：
11.1
通讯作者：
Yuste, R
中科院分区：
文献类型：
--
作者：
Dunaevsky, A;Tashiro, A;Yuste, R
树突棘是哺乳动物中枢神经系统(CNS)中兴奋性输入的突触后部位，其功能尚不清楚。尽管脊柱形态的改变可能介导突触的可塑性，但基底脊髓的运动程度及其调节和功能仍存在争议。我们研究了小鼠中枢神经系统的三个主要神经元：小脑浦肯野细胞、皮质和海马锥体神经元的脊髓运动。用双光子显微镜对急性和培养脑片上绿色荧光蛋白标记的神经元进行时移成像。在所有三种类型的细胞中，树突起(丝状足突和棘突)都是高度动态的，在短时间内表现出多样化的形态重排(
The function of dendritic spines, postsynaptic sites of excitatory input in the mammalian central nervous system (CNS), is still not well understood. Although changes in spine morphology may mediate synaptic plasticity, the extent of basal spine motility and its regulation and function remains controversial. We investigated spine motility in three principal neurons of the mouse CNS: cerebellar Purkinje cells, and cortical and hippocampal pyramidal neurons. Motility was assayed with time-lapse imaging by using two-photon microscopy of green fluorescent protein-labeled neurons in acute and cultured slices. In all three cell types, dendritic protrusions (filopodia and spines) were highly dynamic, exhibiting a diversity of morphological rearrangements over short (