Propagation of structural deviations of poly (amidoamine) fan-shape dendrimers (generations 0-3) characterized by MALDI and electrospray mass spectrometry
Propagation of structural deviations of poly (amidoamine) fan-shape dendrimers (generations 0-3) characterized by MALDI and electrospray mass spectrometry
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DOI：
10.1016/j.ijms.2007.07.002
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发表时间：
2007-10-01
影响因子：
1.8
通讯作者：
Charles, Laurence
中科院分区：
文献类型：
--
作者：
Giordanengo, Remi;Mazarin, Michael;Charles, Laurence
扇形PAMAM树枝状聚合物，从第0代到第3代，通过质谱分析，使用MALDI和电喷雾电离技术，以确定每个样品中存在的任何结构偏差。首先，可以得出结论，所有检测到的分子都存在于样品中，因为它们在MALDI以及电喷雾质谱中被检测到。除了通常报告的树枝状聚合物缺陷（“缺失臂”和“分子环”）外，还发现在合成上一代过程中这些缺陷的传播产生了新的杂质。这些分配是基于化合物的分子量，当离子检测到足够的丰度，从完美的结构行为在MS/MS实验的偏差。由于在每一代新的树枝状聚合物合成过程中，可以从完美或有缺陷的分子中产生新的杂质，因此建立模型以预测化合物的分子量作为其合成历史的函数，并有效地指导质谱解释。(C)2007 Elsevier B. V.保留所有权利。
Fan-shape PAMAM dendrimers, from generations 0 to 3, were analyzed by mass spectrometry, using both MALDI and electrospray ionization techniques, to identify any structural deviations present in each sample. First, it could be concluded that all detected molecules were present in the samples as they were detected in MALDI as well as in electrospray mass spectra. Apart from commonly reported dendrimer defects ("missing arm" and "molecular loop"), new impurities were found to arise from propagation of these defects during the synthesis of upper generations. These assignments were based on both compound molecular weight and, when ions were detected with sufficient abundance, deviations from perfect structure behaviour during MS/MS experiments. Since new impurities could be created, either from perfect or defective molecules, during each new generation dendrimer synthesis, models were built to predict the molecular weight of a compound as a function of its synthesis history and efficiently guide mass spectral interpretation. (C) 2007 Elsevier B.V. All rights reserved.