Tel/PDGFRβ inhibits self-renewal and directs myelomonocytic differentiation of ES cells
Tel/PDGFRβ inhibits self-renewal and directs myelomonocytic differentiation of ES cells
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DOI：
10.1016/j.leukres.2008.02.007
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发表时间：
2008-10-01
影响因子：
2.7
通讯作者：
Wheadon, H.
中科院分区：
文献类型：
--
作者：
Dobbin, E.;Corrigan, P. M.;Wheadon, H.
白血病癌基因TEL/PDGFRβ在胚胎干细胞中被诱导表达，并观察了其在造血分化过程中的表型和分子变化。TEL/PDGFRβ的表达导致ES细胞不能自我更新，导致骨髓生成显著增加，而红细胞生成相应减少。基因表达谱分析表明，在TEL/PDGFRβ表达细胞中，与自我更新和分化相关的基因水平发生了戏剧性的变化，尤其是骨髓单核细胞基因。这项研究表明TEL/PDGFRβ通过改变与造血有关的基因的表达来驱动骨髓生成，并证明了该干细胞系统在研究癌基因诱导的发病机制方面的潜力。(C)2008爱思唯尔有限公司。保留所有权利。
The leukemic oncogene Tel/PDGFR beta, was inducibly expressed in embryonic stem (ES) cells and the phenotypic and molecular changes occurring during hematopoietic differentiation investigated. Expression of Tel/PDGFR beta resulted in an inability of ES cells to self-renew and caused a significant increase in myelopoiesis with a corresponding decrease in erythropoiesis. Analysis of gene expression patterns indicated a dramatic alteration in the levels of genes associated with self-renewal and differentiation, especially myelomonocytic genes in Tel/PDGFR beta-expressing cells. This study indicates Tel/PDGFR beta drives myelopoiesis by altering expression of genes involved in hematopoiesis and demonstrates the potential of this stem cell system to study oncogene-induced pathogenesis. (c) 2008 Elsevier Ltd. All rights reserved.