Cobtorin target analysis reveals that pectin functions in the deposition of cellulose microfibrils in parallel with cortical microtubules.
Cobtorin target analysis reveals that pectin functions in the deposition of cellulose microfibrils in parallel with cortical microtubules.
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DOI：
10.1111/j.1365-313x.2010.04356.x
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发表时间：
2010-11
期刊：
影响因子：
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通讯作者：
A. Yoneda;Takuya Ito;T. Higaki;N. Kutsuna;Tamio Saito;T. Ishimizu;H. Osada;S. Hasezawa;M. Matsui
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文献类型：
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作者：
A. Yoneda;Takuya Ito;T. Higaki;N. Kutsuna;Tamio Saito;T. Ishimizu;H. Osada;S. Hasezawa;M. Matsui
纤维素和果胶是植物初代细胞壁的主要成分，它们的力学性质对细胞的形态发生起着重要的作用。据推测，皮质微管引导纤维素微纤维合成酶沿与微管平行的方向运动，但这种对齐发生的机制尚不清楚。我们之前已经确定了cotorin作为一种抑制剂，干扰皮质微管和新生纤维素微原纤维之间的平行关系。在本研究中，我们寻找了cotorin的蛋白靶点，我们发现果胶甲基酯酶和聚半乳糖醛酸酶的过表达抑制了cotorin诱导的细胞肿胀表型。此外，聚半乳糖醛酸酶处理恢复了纤维素微原纤维在与皮质微管平行方向的沉积，而维生素d蛋白则扰乱了甲基化果胶的分布。这些结果表明，控制果胶的性质对于纤维素微原纤维的沉积和/或维持其与皮质微管平行的取向是重要的。
Cellulose and pectin are major components of primary cell walls in plants, and it is believed that their mechanical properties are important for cell morphogenesis. It has been hypothesized that cortical microtubules guide the movement of cellulose microfibril synthase in a direction parallel with the microtubules, but the mechanism by which this alignment occurs remains unclear. We have previously identified cobtorin as an inhibitor that perturbs the parallel relationship between cortical microtubules and nascent cellulose microfibrils. In this study, we searched for the protein target of cobtorin, and we found that overexpression of pectin methylesterase and polygalacturonase suppressed the cobtorin-induced cell-swelling phenotype. Furthermore, treatment with polygalacturonase restored the deposition of cellulose microfibrils in the direction parallel with cortical microtubules, and cobtorin perturbed the distribution of methylated pectin. These results suggest that control over the properties of pectin is important for the deposition of cellulose microfibrils and/or the maintenance of their orientation parallel with the cortical microtubules.