Novel and Sensitive Chemiluminescence Sensors Based on 2D-MOF Nanosheets for One-Step Detection of Glucose in Human Urine
Novel and Sensitive Chemiluminescence Sensors Based on 2D-MOF Nanosheets for One-Step Detection of Glucose in Human Urine
复制标题
基于 2D-MOF 纳米片的新型灵敏化学发光传感器，用于一步检测人尿液中的葡萄糖
DOI：
10.1021/acs.jpcc.9b00671
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发表时间：
2019
影响因子：
3.7
通讯作者：
Zhang Zhen
中科院分区：
文献类型：
--
作者：
Zhu Nuanfei;Gu Lantian;Wang Jin;Li Xuesong;Liang Guoxi;Zhou Jinhui;Zhang Zhen
建立一种简便、灵敏的血糖测定方法对糖尿病的早期诊断和治疗具有重要意义。然而，目前的分析方法在应用中存在着各种缺陷。在这里，一种引人注目的基于化学发光的传感器被开发出来，用于一步快速检测人体尿液中的葡萄糖。本工作首次合成了具有过氧化物酶活性的二维金属有机骨架(2D-MOF)纳米片，它可以催化过氧化氢(H_2O_2)的分解，进而氧化鲁米诺产生强烈的化学发光信号。然后，将鲁米诺和葡萄糖氧化酶(GOD)先后标记在2D-MOF纳米片上，得到了一种简单的化学发光功能化传感器(Co-TCPP(Fe)@鲁米诺@GOD)。当Co-TCPP(Fe)@鲁米诺@GOD被引入含葡萄糖的检测体系时，在GOD的催化下，Co-TCPP(Fe)上释放出大量的H_2O_2。然后，用2D-MOF纳米薄片分解生成的过氧化氢，并将鲁米诺用于催化反应。
The development of a simple and sensitive method for glucose determination is significant in the early diagnosis and treatment of diabetes. However, current analytical approaches have various defects in their applications. Here, an attractive chemiluminescence-based sensor is developed for one-step and rapid detection of glucose in human urine. In this work, a 2D metal–organic framework (2D-MOF) nanosheet with peroxidase activity was first synthesized, which could catalyze the decomposition of hydrogen peroxide (H2O2) and further oxidize luminol to produce a strong chemiluminescence signal. Then, luminol and glucose oxidase (GOD) were successively labeled on the 2D-MOF nanosheet, obtaining a simple chemiluminescence-functionalized sensor (Co-TCPP(Fe)@luminol@GOD). When Co-TCPP(Fe)@luminol@GOD was introduced to the detection system containing glucose, substantial H2O2 was released under the catalysis of GOD on Co-TCPP(Fe). Then, the produced H2O2 was decomposed by the 2D-MOF nanosheet, and the luminol on C...