A profile of differentially expressed genes in primary colorectal cancer using suppression subtractive hybridization
A profile of differentially expressed genes in primary colorectal cancer using suppression subtractive hybridization
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DOI：
10.1016/s0014-5793(99)01578-1
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发表时间：
1999-12-10
期刊：
影响因子：
3.5
通讯作者：
Hoogenboom, HR
中科院分区：
文献类型：
--
作者：
Hufton, SE;Moerkerk, PT;Hoogenboom, HR
为了进一步了解正常细胞和癌细胞之间的复杂差异，我们利用抑制性消减杂交技术，从35000个克隆的克隆库中筛选了40 0个随机克隆，其中45个克隆在肿瘤组织中高表达，测序显示37个不同的基因，其中16个基因与公共数据库中的已知基因相对应。包括Smad5和Fls353在内的12个基因在结直肠癌中过表达，一系列以前没有报道在癌症中过表达的已知基因也被恢复：Hsc70，PBEF，Riophorin II和Ese-3B，其余21个绅士到目前为止还没有功能注释，这些结果表明SSH结合高通量筛选提供了一个非常有效的手段来获得广泛的癌症差异表达基因。已确定的一些基因可能提供新的治疗干预点，(C)1999年欧洲生化学会联合会。
As a step towards understanding the complex differences between normal cells and cancer cells, we have used suppression subtractive hybridization (SSH) to generate a profile of genes overexpressed in primary colorectal cancer (CRC), From a 35000 clone SSH-cDNA repertoire, we have screened 400 random clones by reverse Northern blotting, of which 45 clones were scored as overexpressed in tumor compared to matched normal mucosa, Sequencing showed 37 different genes and of these, 16 genes corresponded to known genes in the public databases. Twelve genes, including Smad5 and Fls353, have previously been shown to be overexpressed in CRC, A series of known genes which have not previously been reported to be overexpressed in cancer were also recovered: Hsc70, PBEF, ribophorin II and Ese-3B, The remaining 21 gents have as yet no functional annotation, These results show that SSH in conjunction with high throughput screening provides a very efficient means to produce a broad profile of genes differentially expressed in cancer. Some of the genes identified may provide novel points of therapeutic intervention, (C) 1999 Federation of European Biochemical Societies.