Real-time fluorescence lifetime imaging system with a 32 x 32 0.13μm CMOS low dark-count single-photon avalanche diode array
Real-time fluorescence lifetime imaging system with a 32 x 32 0.13μm CMOS low dark-count single-photon avalanche diode array
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DOI：
10.1364/oe.18.010257
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发表时间：
2010-05-10
期刊：
影响因子：
3.8
通讯作者：
Henderson, Robert
中科院分区：
文献类型：
--
作者：
Li, Day-Uei;Arlt, Jochen;Henderson, Robert
一个紧凑的实时荧光寿命成像显微镜（FLIM）系统的基础上的低暗计数0.13 μ m CMOS单光子雪崩二极管（SPAD）的阵列被证明。快速背景不敏感荧光寿命测定通过使用最近提出的称为“提取方法的积分”（IEM）的算法来实现[J. Opt. Soc. Am. A 25，1190（2008）]。在这里，IEM被修改为更宽的分辨率范围，并在新的SPAD阵列成像器的FPGA上实现。我们的实验表明，这种新的相机的动态范围和计算寿命的准确性是适合于宽场FLIM应用成像的各种测试样品，包括各种标准的荧光团覆盖的寿命范围从1.6ns到16ns，荧光团溶液的微流体混合，和活真菌孢子的粗糙脉孢菌。计算的寿命与文献值吻合得很好。实时荧光寿命成像也实现了，通过执行并行的32 × 16寿命计算，实现了紧凑和低成本的FLIM相机和更大的检测器阵列的前景。(c)2010年美国光学学会
A compact real-time fluorescence lifetime imaging microscopy (FLIM) system based on an array of low dark count 0.13 mu m CMOS single-photon avalanche diodes (SPADs) is demonstrated. Fast background-insensitive fluorescence lifetime determination is achieved by use of a recently proposed algorithm called 'Integration for Extraction Method' (IEM) [J. Opt. Soc. Am. A 25, 1190 (2008)]. Here, IEM is modified for a wider resolvability range and implemented on the FPGA of the new SPAD array imager. We experimentally demonstrate that the dynamic range and accuracy of calculated lifetimes of this new camera is suitable for widefield FLIM applications by imaging a variety of test samples, including various standard fluorophores covering a lifetime range from 1.6ns to 16ns, microfluidic mixing of fluorophore solutions, and living fungal spores of Neurospora Crassa. The calculated lifetimes are in a good agreement with literature values. Real-time fluorescence lifetime imaging is also achieved, by performing parallel 32 x 16 lifetime calculations, realizing a compact and low-cost FLIM camera and promising for bigger detector arrays. (c) 2010 Optical Society of America