Molecular profiling of extracellular vesicles via charge-based capture using oxide nanowire microfluidics
Molecular profiling of extracellular vesicles via charge-based capture using oxide nanowire microfluidics
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使用氧化物纳米线微流体通过基于电荷的捕获对细胞外囊泡进行分子分析
DOI：
10.1016/j.bios.2021.113589
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发表时间：
2021
影响因子：
12.6
通讯作者：
Baba Yoshinobu
中科院分区：
文献类型：
--
作者：
Yasui Takao;Paisrisarn Piyawan;Yanagida Takeshi;Konakade Yuki;Nakamura Yuta;Nagashima Kazuki;Musa Marina;Thiodorus Ivan Adiyasa;Takahashi Hiromi;Naganawa Tsuyoshi;Shimada Taisuke;Kaji Noritada;Ochiya Takahiro;Kawai Tomoji;Baba Yoshinobu
细胞外囊泡（EV）已显示出作为早期癌症诊断的生物标志物的有前途的功能。癌细胞衍生的EV的外层由亲器官转移诱导的膜蛋白和特异性富集的蛋白聚糖组成，并且这些分子组成决定EV表面电荷。虽然许多努力已经致力于调查通过密度，大小和免疫亲和性为基础的捕获和表达的膜蛋白获得的EV子集之间的相关性，了解通过表面电荷为基础的捕获和表达的膜蛋白获得的EV子集之间的相关性是缺乏的。在这里，我们提出了一种方法来配置文件通过基于表面电荷的捕获获得的EV子集的膜蛋白。纳米线诱导的基于电荷的EV捕获和EV膜蛋白的原位分析是两个关键的方法学点。氧化物纳米线允许通过基于表面电荷的捕获获得EV，这是由于氧化物的不同等电点和纳米线结构的大的表面积与体积比。而且，使用ZnO纳米线设备，其使用不需要任何纯化和浓缩过程，我们证明了带负电荷的EV子集和来自每个细胞的表达膜蛋白之间的相关性。此外，我们确定结肠癌相关膜蛋白在源自结肠癌细胞的带负电荷的表面EV上过表达。
Extracellular vesicles (EVs) have shown promising features as biomarkers for early cancer diagnoses. The outer layer of cancer cell-derived EVs consists of organotropic metastasis-induced membrane proteins and specifically enriched proteoglycans, and these molecular compositions determine EV surface charge. Although many efforts have been devoted to investigating the correlation between EV subsets obtained through density-, size-, and immunoaffinity-based captures and expressed membrane proteins, understanding the correlation between EV subsets obtained through surface charge-based capture and expressed membrane proteins is lacking. Here, we propose a methodology to profile membrane proteins of EV subsets obtained through surface charge-based capture. Nanowire-induced charge-based capture of EVs andin-situprofiling of EV membrane proteins are the two key methodology points. The oxide nanowires allowed EVs to be obtained through surface charge-based capture due to the diverse isoelectric points of the oxides and the large surface-to-volume ratios of the nanowire structures. And, with the ZnO nanowire device, whose use does not require any purification and concentration processes, we demonstrated the correlation between negatively-charged EV subsets and expressed membrane proteins derived from each cell. Furthermore, we determined that a colon cancer related membrane protein was overexpressed on negatively charged surface EVs derived from colon cancer cells.