Optimization of a Procedure for Extraction of Nucleotides from Plasma and Erythrocytes Prior to HPLC Analysis
Optimization of a Procedure for Extraction of Nucleotides from Plasma and Erythrocytes Prior to HPLC Analysis
复制标题
HPLC 分析前从血浆和红细胞中提取核苷酸的流程优化
DOI：
10.1080/01483917808060015
复制
发表时间：
1978
影响因子：
1.3
通讯作者：
P. Brown
中科院分区：
文献类型：
--
作者：
D. A. Van;P. Brown
Abstract The efficiency of extraction of nucleotides from plasma and erythrocyte samples by means of the Khym TCA1 - amine-FreonR procedure was investigated. Maximum efficiency of recovery was found when TCA concentrations greater than 9% were used to extract the nucleotides of cytosine, adenine and guanine from plasma and erythrocytes. The recovery from the erythrocyte matrix was 20% lower than that from the plasma matrix and 40% lower than the recovery of the same nucleotides from water. It was also found that using the exact amount of amine solution to neutralize the TCA resulted in increased recovery of the nucleotides.
