Semi-permeable nanocapsules of konjac glucomannan-chitosan for enzyme immobilization.
Semi-permeable nanocapsules of konjac glucomannan-chitosan for enzyme immobilization.
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DOI：
10.1016/j.ijpharm.2008.07.026
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发表时间：
2008-11
影响因子：
5.8
通讯作者：
Rui Wang;Bing Xia;Bang‐Jing Li;S. Peng;L. Ding;S. Zhang
中科院分区：
文献类型：
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作者：
Rui Wang;Bing Xia;Bang‐Jing Li;S. Peng;L. Ding;S. Zhang
羧甲基魔芋葡甘聚糖-壳聚糖（CKGM-CS）纳米胶囊是在非常温和的条件下通过静电络合自发制备的，用于固定L-天冬酰胺酶。基质具有半渗透性，允许底物和产物通过并将L-天冬酰胺酶保留在基质中以防止泄漏。细胞样水凝胶基质在水体系中制备，无需有机溶剂和试剂。制备过程不会使酶变性，固定化酶和天然酶的活性非常相似。研究了载酶纳米胶囊的活性、稳定性和特性。结果表明，与天然酶相比，固定化酶具有更好的稳定性和活性。这些研究可能为pH和温度敏感酶的固定化提供新的材料。
Carboxymethyl konjac glucomannan–chitosan (CKGM–CS) nanocapsules, spontaneously prepared under very mild conditions by electrostatic complexation, were used for immobilizing l-asparaginase. The matrix has semi-permeability to allow the substrate and product to pass through and to keep l-asparaginase in the matrix to prevent leaking. The cell-like hydrogel matrix was prepared in aqueous system without organic solvents and reagents. The process of the preparation does not denature the enzyme and the activity of the immobilized and native enzyme is very similar. The activity, stability, and characters of the enzyme-loaded nanocapsules were studied. The results indicated the immobilized enzyme has better stability and activity in contrast to the native enzyme. These studies may supply a new material for the immobilization of pH and temperature-sensitive enzyme.