Enhancement of corneal endothelium wound healing by Rho-associated kinase (ROCK) inhibitor eye drops
Enhancement of corneal endothelium wound healing by Rho-associated kinase (ROCK) inhibitor eye drops
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DOI：
10.1136/bjo.2010.194571
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发表时间：
2011-07-01
影响因子：
4.1
通讯作者：
Kinoshita, Shigeru
中科院分区：
文献类型：
--
作者：
Okumura, Naoki;Koizumi, Noriko;Kinoshita, Shigeru
目的观察Rho相关激酶（Rho-associated kinase，ROCK）抑制剂Y-27632对角膜内皮损伤愈合的影响。然后在存在或不存在10 μ M Y-27632的情况下，在24小时培养期间测定伤口距离。作为体内模型，通过经角膜冷冻损伤日本白色兔的中央角膜内皮，然后局部应用10 mM Y-27632，每日6次，持续48 h。结果Y-27632组培养的角膜内皮细胞的平均伤口距离明显短于对照组。结论ROCK抑制剂Y-27632在体外和体内均能促进角膜内皮损伤愈合。
Aim To demonstrate the efficacy of Rho-associated kinase ( ROCK) inhibitor Y-27632 for corneal endothelial wound healing both in in vitro and in vivo models.Methods As an in vitro model, cultivated cynomolgus monkey corneal endothelial cells were scraped to create a linear defect. The wound distance was then determined during a 24-h culture in the presence or absence of 10 mu M of Y-27632. As an in vivo model, central corneal endothelium of Japanese white rabbits was damaged by transcorneal freezing, then 10 mM of Y-27632 was applied topically six times daily for 48 h. The wound area of the corneal endothelium was evaluated after 48 h.Results The mean wound distance in the cultured corneal endothelial cells was significantly shorter in the Y-27632 group than in the control group. In the rabbit model, the mean wound area of the Y-27632 group was significantly smaller than that of the control group.Conclusion This study demonstrated that ROCK inhibitor Y-27632 promotes corneal endothelial wound healing both in in vitro and in vivo.