Anti-inflammatory effects of soyasapogenol I-αa via downregulation of the MAPK signaling pathway in LPS-induced RAW 264.7 macrophages
Anti-inflammatory effects of soyasapogenol I-αa via downregulation of the MAPK signaling pathway in LPS-induced RAW 264.7 macrophages
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DOI：
10.1016/j.fct.2018.01.050
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发表时间：
2018-03-01
影响因子：
4.3
通讯作者：
Chung, Gyuhwa
中科院分区：
文献类型：
--
作者：
Yang, Seung Hwan;Le, Bao;Chung, Gyuhwa
据报道，大豆皂苷的粗提物具有抗炎活性。我们确定了从野生大豆 (Glycine soja) 中分离出的新纯度 I 组皂苷、I-α a 和 I-gamma a，了解其保护 RAW 264.7 巨噬细胞免受脂多糖 (LPS) 刺激的功效。用大豆皂苷 I-α a/I-gamma a (30-300 μM) 和 LPS (0.1 μg/mL) 处理细胞 24 小时。大豆皂苷 I-α a 在 100 μg/mL 浓度下可抑制一氧化氮 (NO) 的产生，而大豆皂苷 I-gamma a 在较高浓度（200 μg/mL）下则显示出这种作用。两种大豆皂苷均下调 iNOS 和 COX-2 酶的表达水平。大豆皂苷 I-α a 通过 TNF-α 和 IL-1 β 细胞因子发挥作用。然而，大豆皂苷I-γa仅抑制TNF-α的表达。 I 族大豆皂苷的炎症作用主要通过 p38 和 JNK 蛋白的磷酸化介导。总的来说，这些结果表明大豆皂苷具有潜在的抗炎作用。
The crude extract of soyasaponins was reported to possess anti-inflammatory activity. We determined the new purity group I saponin, I-alpha a and I-gamma a that was isolated from wild soybean (Glycine soja) in terms of its efficacy in protecting RAW 264.7 macrophages from lipopolysaccharide (LPS)-stimuli. Cells were treated with soyasaponin I-alpha a/I-gamma a (30-300 mu M) and LPS (0.1 mu g/mL) for 24 h. Soyasaponin I-alpha a inhibited nitric oxide (NO) production at 100 mu g/mL, while soyasaponin I-gamma a demonstrated this effect at a higher concentration (200 mu g/mL). The expression levels of iNOS and COX-2 enzymes were downregulated by both soyasaponins. Soyasaponin I-alpha a exerted its effect via the TNF-alpha and IL-1 beta cytokines. However, soyasaponin I-gamma a only inhibited the expression of TNF-alpha. The inflammatory effect of group I soyasaponin was mainly mediated via the phosphorylation of the p38 and JNK proteins. Collectively, these results suggested the potential anti-inflammatory effects of soyasaponins.